Naseer Zahid, Ahmad Ejaz, Aksoy Melih, Küçük Niyazi, Serin İlker, Ceylan Ahmet, Boyacıoğlu Murat, Kum Cavit
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey; Department of Clinical Sciences, Faculty of Veterinary and Animal Sciences, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey.
Cryobiology. 2015 Aug;71(1):18-23. doi: 10.1016/j.cryobiol.2015.06.007. Epub 2015 Jun 19.
Three experiments were conducted to determine the protective effect of cholesterol-loaded cyclodextrin (CLC) against hydrogen peroxide (H2O2) or cryo-induced damage in ram sperm. In Experiment 1, the fresh ejaculates were either treated with CLC or remained untreated. Both CLC treated and untreated samples were then incubated with 0, 250 or 500 μM H2O2 at 35°C for 12 h. After incubation period of 12 h, the motility, viability and membrane integrity remained higher in CLC treated sperm even in the presence of 250 or 500 μM H2O2. The H2O2 treatment affected all the sperm parameters adversely (P<0.05). However, compared to CLC untreated counterpart, the motility, viability and membrane integrity remained higher (P<0.05) in treated sperm, even in the presence of 250 or 500 μM H2O2 during 12 h of incubation. In Experiment 2, semen was cryopreserved in the presence or absence of CLC. The post-thaw results revealed that CLC treated sperm has higher (P<0.05) motility, viability and membrane integrity compared to the control. In Experiment 3, lipid peroxidation levels were assessed by determining malondialdehyde (MDA) concentrations during the H2O2-induced oxidative stress in CLC treated and untreated sperm. However, no difference (P>0.05) in MDA level was observed among the groups at any stage of incubation. In conclusion, the CLC incorporation in ram sperm membrane may protects it against H2O2 or cryo-induced oxidative damage. The cryoprotective influence of CLC on ram sperm might be resulted from, at least partly, its antioxidative property.
进行了三项实验，以确定胆固醇负载环糊精（CLC）对过氧化氢（H2O2）或冷冻诱导的公羊精子损伤的保护作用。在实验1中，新鲜射精的精液要么用CLC处理，要么不进行处理。然后将经CLC处理和未处理的样品在35°C下与0、250或500μM H2O2孵育12小时。孵育12小时后，即使存在250或500μM H2O2，经CLC处理的精子的活力、存活率和膜完整性仍较高。H2O2处理对所有精子参数均产生不利影响（P<0.05）。然而，与未用CLC处理的对应物相比，即使在孵育12小时期间存在250或500μM H2O2，经处理的精子的活力、存活率和膜完整性仍较高（P<0.05）。在实验2中，精液在有或没有CLC的情况下进行冷冻保存。解冻后的结果显示，与对照组相比，经CLC处理的精子具有更高（P<0.05）的活力、存活率和膜完整性。在实验3中，通过测定经CLC处理和未处理的精子在H2O2诱导的氧化应激期间丙二醛（MDA）浓度来评估脂质过氧化水平。然而，在孵育的任何阶段，各实验组之间均未观察到MDA水平的差异（P>0.05）。总之，将CLC掺入公羊精子膜中可能会保护其免受H2O2或冷冻诱导的氧化损伤。CLC对公羊精子的冷冻保护作用可能至少部分源于其抗氧化特性。
