一种基于曙红Y的用于检测全氟辛烷磺酸的“开启型”荧光传感器。

An eosin Y-based "turn-on" fluorescent sensor for detection of perfluorooctane sulfonate.

作者信息

Liang Jiaman, Deng Xiaoyan, Tan Kejun

机构信息

Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemical and Chemistry Engineering, Southwest University, Chongqing 400715, PR China.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2015;150:772-7. doi: 10.1016/j.saa.2015.05.069. Epub 2015 Jun 6.

DOI:10.1016/j.saa.2015.05.069

PMID:26103430

Abstract

In this paper, a novel sensing method with a higher sensitivity of perfluorooctane sulfonate (PFOS) than perfluorooctanoic acid (PFOA) has been proposed detection of PFOS in aqueous solution replying on the "off-on" switch of eosin Y/polyethyleneimine (PEI)/PFOS fluorescence system due to the higher affinity of PEI to PFOS than eosin Y. In pH 7.0 Britton-Robinson buffer solution, eosin Y reacts with protonated PEI to form complex by electrostatic attraction, which leads to a strong fluorescence quenching of the eosin Y. When PFOS presents, the fluorescence of eosin Y is recover due to the electrostatic and hydrophobic interactions between PFOS and PEI. The recovered fluorescence intensity is proportional to the concentration of PFOS in the ranging from 0 to 2.0×10(-6) mol/L with the limit of detection (LOD, 3σ) being 1.5×10(-8) mol/L without preconcentration. In this study, the optimum reaction conditions and the interferences of foreign substances were investigated. In addition, the effects of PFOA, the analog of PFOS, on the fluorescence recovery of the system were also studied. The presented approach has been successfully used to detect PFOS in real samples with RSD ⩽2.9%.

摘要

本文提出了一种新型传感方法，用于检测水溶液中的全氟辛烷磺酸（PFOS）。该方法基于曙红Y/聚乙烯亚胺（PEI）/PFOS荧光体系的“关-开”开关，对PFOS的灵敏度高于全氟辛酸（PFOA），这是因为PEI对PFOS的亲和力高于曙红Y。在pH 7.0的 Britton-Robinson缓冲溶液中，曙红Y与质子化的PEI通过静电吸引形成复合物，导致曙红Y的荧光强烈猝灭。当存在PFOS时，由于PFOS与PEI之间的静电和疏水相互作用，曙红Y的荧光得以恢复。恢复的荧光强度与PFOS浓度在0至2.0×10⁻⁶ mol/L范围内成正比，无需预富集时的检测限（LOD，3σ）为1.5×10⁻⁸ mol/L。本研究考察了最佳反应条件和外来物质的干扰。此外，还研究了PFOS的类似物全氟辛酸（PFOA）对该体系荧光恢复的影响。所提出的方法已成功用于实际样品中PFOS的检测，相对标准偏差（RSD）≤2.9%。