病原体的表面等离子体共振成像：鼠疫耶尔森菌范例
Surface plasmon resonance imaging of pathogens: the Yersinia pestis paradigm.
作者信息
Huynh Hong T T, Gotthard Guillaume, Terras Jérome, Aboudharam Gérard, Drancourt Michel, Chabrière Eric
机构信息
Faculté de médecine, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR CNRS 7278, IRD 198, INSERM 1095, 27, Boulevard Jean Moulin-Cedex 5, Marseille, France.
出版信息
BMC Res Notes. 2015 Jun 24;8:259. doi: 10.1186/s13104-015-1236-3.
BACKGROUND
Yersinia pestis, causing deadly plague, is classified as a group A bioterrorism bacterium. Some recent DNA-based methods were used for detection of bioterrorism agents.
RESULTS
Y. pestis was used as a model organism to develop an immunosensor based on surface plasmon resonance imaging (SPRi) using monoclonal antibody against Y. pestis F1 antigen. The experimental approach included step-by-step detection of Y. pestis membrane proteins, lysed bacteria, intact bacteria, mock-infected powder and mock-infected clinical specimens. SPRi detected on average 10(6) intact Y. pestis organisms in buffer, in mock-infected powder and in a 1:4 mixture with HEL cells.
CONCLUSIONS
This study offers the proof-of-concept of the SPRi-based detection of a human pathogen in both environmental and clinical specimens.
背景
导致致命鼠疫的鼠疫耶尔森菌被归类为A类生物恐怖主义细菌。最近一些基于DNA的方法被用于检测生物恐怖主义病原体。
结果
鼠疫耶尔森菌被用作模式生物，以开发一种基于表面等离子体共振成像（SPRi）的免疫传感器，该传感器使用针对鼠疫耶尔森菌F1抗原的单克隆抗体。实验方法包括逐步检测鼠疫耶尔森菌膜蛋白、裂解细菌、完整细菌、模拟感染粉末和模拟感染临床标本。SPRi平均检测到缓冲液、模拟感染粉末以及与HEL细胞按1:4混合的样本中10⁶个完整的鼠疫耶尔森菌。
结论
本研究提供了基于SPRi在环境和临床标本中检测人类病原体的概念验证。
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