Ho C K, Ou B R, Wang C Y, Chen H Y, Kuwata T
Department of Medical Research, Veterans General Hospital, Taipei, Taiwan, Republic of China.
Arch Virol. 1989;109(1-2):25-34. doi: 10.1007/BF01310515.
The effect of retinoic acid (RA) on the antiviral activity of interferons (IFNs) alpha and beta in the U937 and WISH cells was examined to ascertain whether or not RA could reduce the effectiveness of IFN-induced resistance to viral infection. Our results indicate that in the U937 cells, RA (0.1-1.0 microM) had neither enhancing nor suppressive effect on the antiviral activity of IFN-alpha or beta against the Semliki Forest virus (SFV). However, in the WISH cells, RA had different effects on IFNs alpha and beta. Thus, while RA (0.1-50 microM) invariably suppressed the activity of IFN-alpha, it enhanced the action of IFN-beta at low dose (0.1-1.0 microM) but became suppressive at higher concentrations (greater than or equal to 10 microM). Furthermore, higher antiviral activity was consistently obtained when RA (0.1-10 microM) was added prior to either IFN-alpha or IFN-beta comparing to cultures with IFN alone. In addition, direct correlation between antiviral activity and the amplitude of 2-5 oligoadenylate (A) synthetase activity was not observed. These results suggest that modulation of IFN antiviral activity by RA varies with different systems and is dependent on the sequence of treatment.
研究了视黄酸（RA）对U937和WISH细胞中干扰素（IFN）α和β抗病毒活性的影响，以确定RA是否会降低IFN诱导的抗病毒感染抗性的有效性。我们的结果表明，在U937细胞中，RA（0.1 - 1.0微摩尔）对IFN-α或β针对Semliki森林病毒（SFV）的抗病毒活性既无增强作用也无抑制作用。然而，在WISH细胞中，RA对IFN-α和β有不同影响。因此，虽然RA（0.1 - 50微摩尔）始终抑制IFN-α的活性，但它在低剂量（0.1 - 1.0微摩尔）时增强IFN-β的作用，而在较高浓度（大于或等于10微摩尔）时则变为抑制作用。此外，与单独使用IFN的培养物相比，当在IFN-α或IFN-β之前添加RA（0.1 - 10微摩尔）时，始终能获得更高的抗病毒活性。另外，未观察到抗病毒活性与2 - 5寡腺苷酸（A）合成酶活性幅度之间的直接相关性。这些结果表明，RA对IFN抗病毒活性的调节因不同系统而异，并且取决于处理顺序。
