Ogawa T, Kitamura K, Kawamoto M, Eto T, Tanaka K
First Department of Internal Medicine, Miyazaki Medical College, Japan.
Biochem Biophys Res Commun. 1989 Dec 29;165(3):1399-405. doi: 10.1016/0006-291x(89)92759-9.
A high concentration of immunoreactive neuropeptide Y was observed in rat platelets using a specific and sensitive radioimmunoassay for neuropeptide Y. Three kinds of high performance liquid chromatography combined with radioimmunoassay for neuropeptide Y showed that immunoreactive neuropeptide Y in rat platelets is identical to rat authentic neuropeptide Y. To investigate the pathological role of platelet neuropeptide Y in genetic hypertensive rats, the platelet content and plasma concentration of neuropeptide Y were measured by a sensitive radioimmunoassay for rat neuropeptide Y in 5-, 10- and 15-wk old spontaneously hypertensive rat and age-matched Wistar Kyoto rat. Platelet content of immunoreactive neuropeptide Y in 5-, 10- and 15-wk old spontaneously hypertensive rat was higher than that in Wistar Kyoto rat at each age. No difference was observed in plasma concentration of immunoreactive neuropeptide Y between spontaneously hypertensive rat and Wistar Kyoto rat at each age.
利用针对神经肽Y的特异性灵敏放射免疫分析法，在大鼠血小板中观察到了高浓度的免疫反应性神经肽Y。三种高效液相色谱法结合神经肽Y放射免疫分析法表明，大鼠血小板中的免疫反应性神经肽Y与大鼠天然神经肽Y相同。为了研究血小板神经肽Y在遗传性高血压大鼠中的病理作用，采用针对大鼠神经肽Y的灵敏放射免疫分析法，测定了5周龄、10周龄和15周龄自发性高血压大鼠以及年龄匹配的Wistar Kyoto大鼠中神经肽Y的血小板含量和血浆浓度。各年龄段5周龄、10周龄和15周龄自发性高血压大鼠血小板中免疫反应性神经肽Y的含量均高于Wistar Kyoto大鼠。各年龄段自发性高血压大鼠和Wistar Kyoto大鼠血浆中免疫反应性神经肽Y的浓度均未观察到差异。
