Cao Shanshan, Song Shanshan, Liu Liqiang, Kong Na, Kuang Hua, Xu Chuanlai
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University , Wuxi, JiangSu , People's Republic of China.
Immunol Invest. 2015;44(5):438-50. doi: 10.3109/08820139.2015.1021354.
An enzyme-linked immunosorbent assay (ELISA) and an immunochromatographic assay were constructed for the detection of lincomycin (LIN) in both milk and honey samples based on the monoclonal antibody named 5F6. The half-maximum inhibition of ELISA was 0.3 ng/mL after optimizing pH and ionic strength conditions; the limit of detection was 0.07 ng/mL. The cross-reactivity with clindamycin was 0.6%. LIN recovery in spiked milk and honey samples ranged from 84.6% to 115.6% with intra-assay coefficient variations of 1.7-25.4% and inter-assay coefficient variations of 2.7-8.9%. The detection limits were estimated as 2.1 µg/L for milk and 2.1 µg/kg for honey samples. The immunochromatographic assay revealed a LIN cut-off value of 10 ng/mL in PBS, 5 ng/mL in milk, and 120 ng/g in honey, and a visual lower detection limit of 2.5 ng/mL, 1 ng/mL and 30 ng/g in PBS, milk and honey, respectively. The immunochromatographic assay is preferred for large-scale practical application for its simpler pretreatment and satisfied sensitivity compared with ELISA assay.
基于名为5F6的单克隆抗体，构建了酶联免疫吸附测定法（ELISA）和免疫层析测定法，用于检测牛奶和蜂蜜样品中的林可霉素（LIN）。优化pH和离子强度条件后，ELISA的半数抑制浓度为0.3 ng/mL；检测限为0.07 ng/mL。与克林霉素的交叉反应率为0.6%。加标牛奶和蜂蜜样品中LIN的回收率在84.6%至115.6%之间，批内变异系数为1.7 - 25.4%，批间变异系数为2.7 - 8.9%。牛奶样品的检测限估计为2.1 μg/L，蜂蜜样品的检测限为2.1 μg/kg。免疫层析测定法显示，在PBS中LIN的截断值为10 ng/mL，在牛奶中为5 ng/mL，在蜂蜜中为120 ng/g，在PBS、牛奶和蜂蜜中的目视下限检测限分别为2.5 ng/mL、1 ng/mL和30 ng/g。与ELISA测定法相比，免疫层析测定法预处理更简单，灵敏度也令人满意，因此更适合大规模实际应用。
