Shang Yuhan, Zhang Dandan, Shen Yun, Pan Yuanhu, Wang Jing, Wang Yulian
National Reference Laboratory of Veterinary Drug Residues (HZAU), Huazhong Agricultural University, Wuhan 430070, China.
National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, China.
Foods. 2024 Sep 29;13(19):3118. doi: 10.3390/foods13193118.
Prolonged consumption of animal-derived foods containing high levels of lincomycin (LIN) residues can adversely impact human health. Therefore, it is essential to develop specific antibodies and immunoassay methods for LIN. This study utilized computational chemistry to predict the efficacy of LIN haptens prior to chemical synthesis, with subsequent confirmation obtained through an immunization experiment. A hybridoma cell line named LIN/1B11 was established, which is specific to LIN. The optimized indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method exhibited high specificity for detecting LIN residues, with an IC50 value of 0.57 ± 0.03 µg/kg. The method effectively detected LIN residues in pork and milk samples, achieving a limit of detection (LOD) ranging from 0.81 to 1.20 µg/kg and a limit of quantification (LOQ) ranging from 2.09 to 2.29 µg/kg, with recovery rates between 81.9% and 108.8%. This study offers a valuable tool for identifying LIN residues in animal-derived food products. Furthermore, the efficient hapten prediction method presented herein improves antibody preparation efficiency and provides a simple method for researchers in screening haptens.
长期食用含有高含量林可霉素（LIN）残留的动物源性食品会对人体健康产生不利影响。因此，开发针对LIN的特异性抗体和免疫分析方法至关重要。本研究利用计算化学在化学合成前预测LIN半抗原的效果，随后通过免疫实验进行确认。建立了一种名为LIN/1B11的杂交瘤细胞系，它对LIN具有特异性。优化后的间接竞争酶联免疫吸附测定（ic-ELISA）方法在检测LIN残留方面表现出高特异性，IC50值为0.57±0.03µg/kg。该方法有效地检测了猪肉和牛奶样品中的LIN残留，检测限（LOD）为0.81至1.20µg/kg，定量限（LOQ）为2.09至2.29µg/kg，回收率在81.9%至108.8%之间。本研究为识别动物源性食品中的LIN残留提供了一个有价值的工具。此外，本文提出的高效半抗原预测方法提高了抗体制备效率，并为研究人员筛选半抗原提供了一种简单的方法。
