Charpin C, Habib M C, Piana L, Rampal M, Andrac L, Vacheret H, Martini P, Perez-Castillo M, Lavaut M N, Toga M
Laboratoire d'Anatomie Pathologique et Neuropathologie, Faculté de Médecine Timone, Marseille.
Ann Pathol. 1989;9(5):331-9.
The practical applications of computer-assisted image analysis systems are multiple in oncology. The computerized system of image analysis referred to as SAMBA (TITN) is particularly relevant to analyse coloured images resulting from immunostaining or histochemical procedures assessed either on tissue sections of any type or cytological preparations (imprints, smears). The SAMBA analysis of positive Ki67 surfaces in tissue sections from breast, endometrial, ovarian, cervical or urinary bladder samples enables a multiparametric evaluation of the growth fraction (GF) in intraepithelial, borderline or invasive proliferations. Moreover, the SAMBA analysis after Feulgen staining procedures provides a parametric evaluation of the nuclei densitometry and morphological features and of the chromatin texture, which serve to compute the ploidy balance (BP) and the proliferation index (PI). In benign tumors, GF and PI are low and tumor cells are diploid with an overall high positive PB values. In malignant tumors, GF, PI and the percentage of aneuploid cells increase with tumor grade and stage whereas PB decreases. In borderline proliferations, FC, PI and PB intermediate values are recorded. These new criteria of prognosis should be assessed routinely in pathology departments and the results from these new investigations are likely to be soon implicated in the selection of patient therapy.
计算机辅助图像分析系统在肿瘤学中有多种实际应用。被称为SAMBA（TITN）的图像分析计算机系统对于分析通过免疫染色或组织化学程序获得的彩色图像特别有用，这些图像可在任何类型的组织切片或细胞学标本（印片、涂片）上进行评估。对来自乳腺、子宫内膜、卵巢、宫颈或膀胱样本的组织切片中的Ki67阳性表面进行SAMBA分析，能够对上皮内、交界性或浸润性增殖中的生长分数（GF）进行多参数评估。此外，福尔根染色程序后的SAMBA分析可对细胞核密度测定、形态特征和染色质纹理进行参数评估，这些用于计算倍体平衡（BP）和增殖指数（PI）。在良性肿瘤中，GF和PI较低，肿瘤细胞为二倍体，PB总体阳性值较高。在恶性肿瘤中，GF、PI和非整倍体细胞百分比随肿瘤分级和分期增加，而PB降低。在交界性增殖中，记录到FC、PI和PB的中间值。这些新的预后标准应在病理科常规评估，这些新研究的结果可能很快会用于患者治疗的选择。
