Ahmed Atallah F, Al-Yousef Hanan M, Al-Qahtani Jawaher H, Al-Said Mansour S, Ashour AbdelKader E, Al-Sohaibani Mohammed, Rafatullah Syed
Department of Pharmacognosy, College of Pharmacy, King Saud University (KSU), Riyadh, Saudi Arabia ; Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
Department of Pharmacognosy, College of Pharmacy, King Saud University (KSU), Riyadh, Saudi Arabia.
Pharmacogn Mag. 2015 May;11(Suppl 1):S173-81. doi: 10.4103/0973-1296.157726.
Antioxidant natural products and chemoprevention are considered nowadays as an effective approach against health various disorders and diseases induced by oxidative stress or free radicals.
The aim of this study was to assess the hepato- and nephroprotective activity of a standardized red vine leaf aqueous extract AS195 (Antistax(®)).
The protective activity of AS195 (100 mg/kg) was investigated on carbon tetrachloride (CCl4)-intoxicated rats in comparison with silymarin. The flavonoid/proanthocyanidin nature of AS195 was identified by phytochemical and nuclear magnetic resonance (NMR) analyses, while its total phenol/proanthocyanidin/flavonoid content and antioxidant activity were determined by Folin-Ciocalteau, vanillin-sulfuric acid, AlCl3, and 2, 2-diphenyl-2-picrylhydrazyl radical scavenging assays, respectively.
Relative to the control CCl4 -intoxicated group, pretreatment with AS195 could significantly suppressed the elevated serum levels of alanine aminotransferase, alkaline phosphatase, γ-glutamyl transferase, total cholesterol, low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol, triglycerides, bilirubin, creatinine, uric acid, and calcium, whereas it significantly increased the diminished serum levels of high-density lipoprotein cholesterol, albumin and total protein. Moreover, AS195 significantly decreased malondialdehyde formation in the tissues of liver and kidney, whereas it significantly elevated and nonprotein sulfhydryl groups, compared with the intoxicated control. The improvement in biochemical parameters by AS195 was obviously observed and further confirmed by restoration of normal histological features in the two organs.
The results of the present study revealed the capacity of AS195 to enhance the recovery from xenobiotic-induced hepatorenal toxicity initiated by free radicals.
如今，抗氧化天然产物和化学预防被认为是对抗由氧化应激或自由基引起的各种健康紊乱和疾病的有效方法。
本研究旨在评估标准化红葡萄叶水提取物AS195（Antistax®）的肝保护和肾保护活性。
与水飞蓟宾相比，研究了AS195（100 mg/kg）对四氯化碳（CCl4）中毒大鼠的保护活性。通过植物化学和核磁共振（NMR）分析确定了AS195的黄酮类/原花青素性质，同时分别通过福林-西奥尔特法、香草醛-硫酸法、氯化铝法和2,2-二苯基-1-苦基肼自由基清除试验测定了其总酚/原花青素/黄酮含量和抗氧化活性。
相对于CCl4中毒对照组，AS195预处理可显著抑制丙氨酸转氨酶、碱性磷酸酶、γ-谷氨酰转移酶、总胆固醇、低密度脂蛋白胆固醇、极低密度脂蛋白胆固醇、甘油三酯、胆红素、肌酐、尿酸和钙的血清水平升高，而显著提高高密度脂蛋白胆固醇、白蛋白和总蛋白降低的血清水平。此外，与中毒对照组相比，AS195显著降低了肝脏和肾脏组织中丙二醛的形成，而显著提高了非蛋白巯基水平。AS195对生化参数的改善明显可见，并通过两个器官正常组织学特征的恢复得到进一步证实。
本研究结果揭示了AS195增强从自由基引发的外源化学物诱导的肝肾毒性中恢复的能力。
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