环戊二烯基糖缀合物作为凝集素配体和金属诱导的淀粉样蛋白聚集的保护剂。

Cyclam glycoconjugates as lectin ligands and protective agents of metal-induced amyloid aggregation.

作者信息

Lanza Valeria, D'Agata Roberta, Iacono Giuseppe, Bellia Francesco, Spoto Giuseppe, Vecchio Graziella

机构信息

Istituto di Biostrutture e Bioimmagini (IBB), CNR, via P. Gaifami 18, 95125 Catania, Italy.

Dipartimento di Scienze Chimiche, University of Catania Viale A. Doria 6, 95125 Catania, Italy.

出版信息

J Inorg Biochem. 2015 Dec;153:377-382. doi: 10.1016/j.jinorgbio.2015.06.016. Epub 2015 Jun 19.

DOI:10.1016/j.jinorgbio.2015.06.016

PMID:26112151

Abstract

Transition metal ion complexes of a number of chelators have been widely investigated due to their biological properties. The sugar conjugation of metal complexes has resulted in improved properties of the systems, such as solubility and lectin recognition. In this paper, we report the synthesis, the characterization of new glucose and galactose conjugates of 1,4,8,11-tetraazacyclotetradecane (cyclam) and their Cu(II) complexes. The glycoconjugates were proved to be protective agents of metal-induced amyloid aggregation. The binding constants of the galactose conjugate KA1=(3.07±0.16)×10(7) and KA2=(2.13±0.04)×10(7)M(-1) with the lectin Ricinus communis agglutinin (RCA120) as a model of galactose-specific human lectin were obtained with surface plasmon resonance.

摘要

由于具有生物特性，多种螯合剂的过渡金属离子配合物已得到广泛研究。金属配合物的糖缀合作用改善了体系的性质，如溶解性和凝集素识别性。本文报道了1,4,8,11-四氮杂环十四烷（环胺）新的葡萄糖和半乳糖缀合物及其铜（II）配合物的合成与表征。糖缀合物被证明是金属诱导淀粉样聚集的保护剂。以半乳糖特异性人凝集素蓖麻凝集素（RCA120）为模型，通过表面等离子体共振得到了半乳糖缀合物与RCA120的结合常数KA1=(3.07±0.16)×10(7)和KA2=(2.13±0.04)×10(7)M(-1)。