Zahm J M, Pierrot D, Vaquez-Girod S, Duvivier C, King M, Puchelle E
INSERM U314, Faculté de Médecine, Reims, France.
Biorheology. 1989;26(4):747-52. doi: 10.3233/bir-1989-26407.
Using a simulated cough machine, we analyzed the effect of adding tensio-active liquids as sol phase simulant on the clearance of gel mucus simulant by cough. Polysaccharides crosslinked with sodium tetraborate were used at different concentration as gel mucus simulant. A drop of gel mucus simulant was deposited either directly on the model trachea or on a sol phase layer simulant (2% sodium dodecyl sulfate in water). The clearance of the mucus simulants was quantified by observing the movement of marker particles in the gel layer. The viscoelastic properties of gel mucus simulants were determined by using a viscoelastometer (SEFAM). The adhesive properties were analyzed by means of the platinum ring technique. The wettability of the mucus simulants was quantified by the automatic measurement of the contact angle of the drop of gel on the model trachea. We found that the addition of a sol phase significantly decreased by about 50% the adhesivity and wettability of the gel mucus simulants. This decrease was associated with a marked enhancement of cough clearance, whatever the viscoelastic properties of the gel mucus simulants. These results suggest that the sol phase is essential in bronchial respiratory mucus clearance by the cough mechanism.
我们使用模拟咳嗽机，分析了添加表面活性液体作为液相模拟物对咳嗽清除凝胶黏液模拟物的影响。用与四硼酸钠交联的多糖在不同浓度下作为凝胶黏液模拟物。将一滴凝胶黏液模拟物直接滴在模型气管上或滴在液相层模拟物（水中2%的十二烷基硫酸钠）上。通过观察凝胶层中标记颗粒的移动来量化黏液模拟物的清除情况。使用粘弹性仪（SEFAM）测定凝胶黏液模拟物的粘弹性特性。通过铂环技术分析其黏附特性。通过自动测量凝胶滴在模型气管上的接触角来量化黏液模拟物的润湿性。我们发现，添加液相可使凝胶黏液模拟物的黏附性和润湿性显著降低约50%。无论凝胶黏液模拟物的粘弹性特性如何，这种降低都与咳嗽清除的显著增强有关。这些结果表明，液相在咳嗽机制清除支气管呼吸道黏液中至关重要。
