聋动物耳蜗中减弱的红外神经元刺激反应可能与螺旋神经节神经元的退化有关。
Attenuated infrared neuron stimulation response in cochlea of deaf animals may associate with the degeneration of spiral ganglion neurons.
作者信息
Xie Bingbin, Dai Chunfu, Li Huawei
机构信息
Department of Otology and Skull Base Surgery, Hearing Research Key Lab of Health Ministry of China, Eye and ENT Hospital, Fudan University, Shanghai, 200031, China.
Department of Otology and Skull Base Surgery, Hearing Research Key Lab of Health Ministry of China, Eye and ENT Hospital, Fudan University, Shanghai, 200031, China ;
出版信息
Biomed Opt Express. 2015 May 7;6(6):1990-2005. doi: 10.1364/BOE.6.001990. eCollection 2015 Jun 1.
HYPOTHESIS
We hypothesize that degenerated spiral ganglion neurons (SGNs) in guinea pigs reduces auditory brainstem responses evoked by pulsed infrared stimulation.
BACKGROUND
Pulsed infrared laser excitation can directly evoke physiological responses in neuronal and other excitable cells in vivo and in vitro. Laser pulses could benefit patients with cochlear implants to stimulate the auditory system.
METHODS
Pulsed infrared lasers were used to study evoked optical auditory brainstem responses (oABRs) in normal hearing and deafened animals. Aslo, the morphology and anatomy of SGNs in normal hearing and deafened guinea pigs were compared.
RESULTS
By recording oABRs evoked by varying infrared laser pulse durations, it is suggested that degeneration of SGNs in deafened guinea pigs was associated with an elevated oABR threshold and with lower amplitudes. Moreover, oABR threshold decreased while amplitudes increased in both normal hearing and deafened animals as the pulse duration prolonged. Electron microscopy revealed that SGNs in deafened guinea pigs had swollen and vacuolar mitochondria, as well as demyelinated soma and axons.
CONCLUSION
Infrared laser pulses can stimulate SGNs to evoke oABRs in guinea pigs. Deafened guinea pigs have elevated thresholds and smaller amplitude responses, likely a result of degenerated SGNs. Short pulse durations are more suitable to evoke responses in both normal hearing and deafened animals.
假设
我们推测豚鼠中退化的螺旋神经节神经元（SGNs）会降低脉冲红外刺激诱发的听觉脑干反应。
背景
脉冲红外激光激发可在体内和体外直接诱发神经元及其他可兴奋细胞的生理反应。激光脉冲可能有益于人工耳蜗植入患者以刺激听觉系统。
方法
使用脉冲红外激光研究正常听力和耳聋动物的诱发光学听觉脑干反应（oABRs）。此外，比较了正常听力和耳聋豚鼠中SGNs的形态和解剖结构。
结果
通过记录不同红外激光脉冲持续时间诱发的oABRs，提示耳聋豚鼠中SGNs的退化与oABR阈值升高和振幅降低有关。此外，随着脉冲持续时间延长，正常听力和耳聋动物的oABR阈值均降低而振幅均增加。电子显微镜显示，耳聋豚鼠中的SGNs有线粒体肿胀和空泡化，以及胞体和轴突脱髓鞘。
结论
红外激光脉冲可刺激豚鼠中的SGNs诱发oABRs。耳聋豚鼠的阈值升高且反应振幅较小，可能是SGNs退化的结果。短脉冲持续时间更适合在正常听力和耳聋动物中诱发反应。
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