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意大利市售生的和即食绿叶蔬菜的微生物学调查。

Microbiological survey of raw and ready-to-eat leafy green vegetables marketed in Italy.

机构信息

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER), Via Bianchi no. 9, 25124 Brescia, Italy.

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER), Via Bianchi no. 9, 25124 Brescia, Italy.

出版信息

Int J Food Microbiol. 2015 Oct 1;210:88-91. doi: 10.1016/j.ijfoodmicro.2015.05.026. Epub 2015 Jun 2.

Abstract

The presence of foodborne pathogens (Salmonella spp., Listeria monocytogenes, Escherichia coli O157:H7, thermotolerant Campylobacter, Yersinia enterocolitica and norovirus) in fresh leafy (FL) and ready-to-eat (RTE) vegetable products, sampled at random on the Italian market, was investigated to evaluate the level of risk to consumers. Nine regional laboratories, representing 18 of the 20 regions of Italy and in which 97.7% of the country's population resides, were involved in this study. All laboratories used the same sampling procedures and analytical methods. The vegetable samples were screened using validated real-time PCR (RT-PCR) methods and standardized reference ISO culturing methods. The results show that 3.7% of 1372 fresh leafy vegetable products and 1.8% of 1160 "fresh-cut" or "ready-to-eat" (RTE) vegetable retailed in supermarkets or farm markets, were contaminated with one or more foodborne pathogens harmful to human health.

摘要

本研究旨在调查意大利市场上随机抽取的新鲜叶菜(FL)和即食(RTE)蔬菜产品中食源性致病菌(沙门氏菌、单增李斯特菌、大肠杆菌 O157:H7、耐热性弯曲杆菌、肠侵袭性大肠杆菌和诺如病毒)的存在情况,评估其对消费者的风险水平。共有 9 个地区实验室参与了这项研究,它们代表了意大利的 20 个地区中的 18 个地区,这些地区居住着全国 97.7%的人口。所有实验室均采用相同的采样程序和分析方法。使用经过验证的实时 PCR(RT-PCR)方法和标准化参考 ISO 培养方法对蔬菜样本进行筛选。结果表明,在超市或农贸市场销售的 1372 份新鲜叶菜产品中有 3.7%,1160 份“新鲜切割”或“即食”(RTE)蔬菜中有 1.8%受到一种或多种对人类健康有害的食源性致病菌污染。

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