伴刀豆球蛋白A交叉亲和免疫电泳及图像分析用于半定量评估酗酒者和正常人血清转铁蛋白的微异质性图谱
Concanavalin A crossed affinity immunoelectrophoresis and image analysis for semiquantitative evaluation of microheterogeneity profiles of human serum transferrin from alcoholics and normal individuals.
作者信息
Heegaard N H, Hagerup M, Thomsen A C, Heegaard P M
机构信息
Protein Laboratory, University of Copenhagen, Denmark.
出版信息
Electrophoresis. 1989 Dec;10(12):836-40. doi: 10.1002/elps.1150101207.
The microheterogeneity profile of human serum transferrin from normal and alcoholic subjects was investigated qualitatively and quantitatively by means of Concanavalin A crossed affinity immunoelectrophoresis and an image analysis program. Differences in amounts of nonreacting transferrin molecules were found, suggesting an increase in triantennary glycosylation of transferrin from alcoholics compared with normal individuals. The increased amount of a highly retarded fraction in crude sera from alcoholics was demonstrated to be artefactual, probably due to entrapment or coprecipitation as the fraction disappeared after repeating the analysis with immunosorbent-purified transferrin. In conclusion, affinity electrophoresis represents a simple approach for demonstration of variations in the neutral monosaccharides of glycans and can discriminate between transferrin from alcoholics and normal individuals.
通过伴刀豆球蛋白A交叉亲和免疫电泳和图像分析程序，对正常人和酗酒者的人血清转铁蛋白的微异质性进行了定性和定量研究。发现无反应转铁蛋白分子数量存在差异，这表明与正常个体相比，酗酒者转铁蛋白的三触角糖基化增加。酗酒者粗血清中高度滞留部分增加的量被证明是人为造成的，可能是由于截留或共沉淀，因为在用免疫吸附纯化的转铁蛋白重复分析后该部分消失了。总之，亲和电泳是一种展示聚糖中性单糖变化的简单方法，并且可以区分酗酒者和正常个体的转铁蛋白。
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