Vassart G, Georges M, Monsieur R, Brocas H, Lequarre A S, Christophe D
Science. 1987 Feb 6;235(4789):683-4. doi: 10.1126/science.2880398.
The term "DNA fingerprint" has been used to describe the extensive restriction fragment length polymorphism associated with hypervariable minisatellites present in the human genome. Until now, it was necessary to hybridize Southern blots to specific probes cloned from human genomic DNA in order to obtain individual-specific restriction patterns. The present study describes the surprising finding that the insert-free, wild-type M13 bacteriophage detects hypervariable minisatellites in human and in animal DNA, provided no competitor DNA is used during hybridization. The effective sequence in M13 was traced to two clusters of 15-base pair repeats within the protein III gene of the bacteriophage. This unexpected use of M13 renders the DNA fingerprinting technology more readily available to molecular biology laboratories.
“DNA指纹”一词已被用于描述与人类基因组中存在的高变微卫星相关的广泛限制性片段长度多态性。到目前为止，为了获得个体特异性的限制性图谱，有必要将Southern印迹与从人类基因组DNA克隆的特定探针进行杂交。本研究描述了一个惊人的发现：无插入片段的野生型M13噬菌体可检测人类和动物DNA中的高变微卫星，前提是杂交过程中不使用竞争DNA。M13中的有效序列可追溯到噬菌体蛋白质III基因内的两个15个碱基对重复序列簇。M13的这种意外用途使DNA指纹技术更容易被分子生物学实验室所使用。
