Cotter F, Nasipuri S, Lam G, Young B D
ICRF Medical Oncology Laboratory, St. Bartholomew's Hospital, London, United Kingdom.
Genomics. 1989 Oct;5(3):470-4. doi: 10.1016/0888-7543(89)90011-6.
A new approach to gene mapping which combines enzymatic amplification with high-resolution flow sorting of human chromosomes has been devised. Reliable amplification from as few as 200 chromosomes has been demonstrated. This method, with particular application to mapping the position of chromosomal translocations, has been used to show that the breakpoint for the constitutional translocation t(11;22)(q23;q11) lies proximal to the genes c-ets-1, Thy-1, and T3 delta and distal to the int-2 gene. The mapping was confirmed by Southern analysis to much larger numbers of chromosomes sorted from the same cell line. Control reactions for the bcl-2 gene on chromosome 18 and the C alpha gene of the IGH locus on chromosome 14 demonstrated the discrimination which can be achieved.
一种将酶促扩增与人类染色体的高分辨率流式分选相结合的基因定位新方法已经设计出来。已经证明从少至200条染色体进行可靠的扩增是可行的。这种方法特别适用于绘制染色体易位的位置，已被用于表明结构性易位t(11;22)(q23;q11)的断点位于原癌基因c-ets-1、Thy-1和T3δ基因的近端，而在int-2基因的远端。通过Southern分析对从同一细胞系中分选出来的大量染色体进行检测，证实了该定位。对18号染色体上的bcl-2基因和14号染色体上IGH基因座的Cα基因进行的对照反应表明可以实现区分。
