Vigh G, Quintero G, Farkas G
Chemistry Department, Texas A & M University, College Station 77843-3255.
J Chromatogr. 1989 Dec 22;484:237-50. doi: 10.1016/s0021-9673(01)88971-8.
The retention behaviour of several charged and uncharged solutes on beta-cyclodextrin-silica was studied as a function of the methanol concentration, ionic strength and pH of the eluent in order to develop efficient displacement chromatographic separations for positional and geometric isomers. These retention curves were used to predict the eluent (carrier solvent) compositions that result in solute retentions in excess of k' = 10. The adsorption isotherms of several cationic detergents were determined in these carrier solutions and were found to be convex. The adsorption isotherms of several positional isomers used as test solutes were also determined in these carrier solutions. The adsorption isotherms permitted the development of efficient displacement chromatographic separations for the isomers tested. Column loadings as high as 58 mg were achieved on a regular 4.6 mm I.D. analytical-scale cyclodextrin silica columns.
研究了几种带电和不带电溶质在β-环糊精-硅胶上的保留行为，作为洗脱液中甲醇浓度、离子强度和pH的函数，以便为位置异构体和几何异构体开发高效的置换色谱分离方法。这些保留曲线用于预测导致溶质保留超过k' = 10的洗脱液（载体溶剂）组成。在这些载体溶液中测定了几种阳离子洗涤剂的吸附等温线，发现其为凸形。还在这些载体溶液中测定了用作测试溶质的几种位置异构体的吸附等温线。吸附等温线允许为测试的异构体开发高效的置换色谱分离方法。在常规内径4.6 mm的分析规模环糊精硅胶柱上实现了高达58 mg的柱负载量。
