Vigh G, Quintero G, Farkas G
Chemistry Department, Texas A&M University, College Station 77843-3255.
J Chromatogr. 1990 May 11;506:481-93. doi: 10.1016/s0021-9673(01)91602-4.
The feasibility of preparative enantiomer separations by displacement chromatography on analytical-scale beta-cyclodextrin-silica columns, operated in the reversed-phase mode, was demonstrated using the enantiomers of mephobarbital, hexobarbital, dansylleucine, dansylvaline and dansylphenylalanine as model solutes. The method development scheme (which relies on the determination of the elution-mode retention behavior and the adsorption isotherms of the solutes and the candidate displacers) described in Parts I and II can be used to select the appropriate displacers and the conditions leading to a successful displacement chromatographic separation. The importance of the displacer (both type and concentration) to the success of the displacement chromatographic separation is demonstrated.
以美索比妥、己巴比妥、丹磺酰亮氨酸、丹磺酰缬氨酸和丹磺酰苯丙氨酸的对映体作为模型溶质，证明了在反相模式下操作的分析规模β-环糊精-硅胶柱上，通过置换色谱法进行制备性对映体分离的可行性。第一部分和第二部分中描述的方法开发方案（该方案依赖于溶质和候选置换剂的洗脱模式保留行为以及吸附等温线的测定）可用于选择合适的置换剂和实现成功置换色谱分离的条件。证明了置换剂（类型和浓度）对置换色谱分离成功的重要性。
