Rapid high-performance liquid chromatographic methods for the determination of overdose concentrations of some non-steroidal anti-inflammatory drugs in plasma or serum.

Separate methods are described for the determination of the non-steroidal anti-inflammatory drugs diflunisal, indomethacin, fenoprofen, ibuprofen, ketoprofen, naproxen, mefenamic acid and piroxicam at overdose concentrations in human plasma or serum, using high-performance liquid chromatography and ultraviolet detection. A common extraction, involving protein precipitation with acetonitrile, is employed for all methods. A 25 cm Hypersil ODS (5 mu particle size) analytical column is used for all chromatographic separations, with a mobile phase of acetonitrile-acetate buffer (pH 4.2 or 4.8). The methods are all reproducible and can also determine concentrations that fall within the normal therapeutic range for each drug.