Múnera-Jaramillo M I, Botero-Garcés S
Clinical Pharmacology Unit, Corporación para Investigaciones Biológicas C.I.B., Medellin, Colombia.
J Chromatogr. 1993 Jul 2;616(2):349-52. doi: 10.1016/0378-4347(93)80407-u.
A high-performance liquid chromatographic technique with a lower detection limit for measuring tenoxicam plasma levels was standardized. Tenoxicam was extracted with dichloromethane from acidified plasma and the evaporated extracts were analysed on a reversed-phase column using a methanol-phosphate buffer mobile phase and setting ultraviolet detection at 355 nm. A reproducible calibration curve in the 5-2000 ng/ml range was obtained. The mean recovery of extraction was 98.99 +/- 4.3% and the detection limit was 5 ng/ml.
