Pianezzola E, Bellotti V, Fontana E, Moro E, Gal J, Desai D M
Farmitalia Carlo Erba, Research and Development, Milan, Italy.
J Chromatogr. 1989 Oct 27;495:205-14. doi: 10.1016/s0378-4347(00)82622-5.
The enantiomers of salsolinol were completely separated as diastereoisomeric derivatives, after reaction with S-1-(1-naphthyl)ethyl isothiocyanate, by reversed-phase high-performance liquid chromatography and quantified by electrochemical detection. Good calibration curves were obtained for the quantification and determination of the enantiomeric composition of salsolinol in human urine. The sensitivity and specificity to the assay also permit the determination of the enantiomeric composition of salsolinol in food such as dried bananas and chocolate.
与S-1-(1-萘基)乙基异硫氰酸酯反应后，消旋去甲骆驼蓬碱的对映体作为非对映异构体衍生物通过反相高效液相色谱法完全分离，并通过电化学检测进行定量。获得了用于定量和测定人尿中消旋去甲骆驼蓬碱对映体组成的良好校准曲线。该测定方法的灵敏度和特异性还允许测定干香蕉和巧克力等食物中消旋去甲骆驼蓬碱的对映体组成。
