Deng Y, Maruyama W, Dostert P, Takahashi T, Kawai M, Naoi M
Department of Applied Chemistry, Nagoya Institute of Technology, Japan.
J Chromatogr B Biomed Appl. 1995 Aug 4;670(1):47-54. doi: 10.1016/0378-4347(95)00136-7.
A new method for the quantitative determination of the enantiomers of salsolinol and N-methylsalsolinol, biologically important alkaloids, is reported. The enantiomers were completely separated without derivatization, using a cyclodextrin-modified silica gel column with an HPLC-electrochemical detection system. The HPLC conditions were examined for the best resolution. The method was sensitive enough to detect salsolinol and N-methylsalsolinol at a concentration of less than 0.1 pmol per injection. In the product of the Pictet-Spengler reaction of acetaldehyde with dopamine or epinine, almost equimolar (R)- and (S)-enantiomers of salsolinol and N-methylsalsolinol were detected. Preliminary results indicate that the (R)-enantiomer of both isoquinoline derivatives predominate in the human brain.
报道了一种定量测定生物活性重要生物碱萨索林醇和N-甲基萨索林醇对映体的新方法。使用带有高效液相色谱-电化学检测系统的环糊精修饰硅胶柱，无需衍生化即可完全分离对映体。考察了高效液相色谱条件以实现最佳分离度。该方法灵敏度足够高，每次进样浓度低于0.1皮摩尔时即可检测到萨索林醇和N-甲基萨索林醇。在乙醛与多巴胺或去甲肾上腺素的Pictet-Spengler反应产物中，检测到几乎等摩尔的萨索林醇和N-甲基萨索林醇的（R）-和（S）-对映体。初步结果表明，这两种异喹啉衍生物的（R）-对映体在人类大脑中占主导地位。
