Ardjomandi N, Duttenhoefer F, Xavier S, Oshima T, Kuenz A, Sauerbier S
Department of Oral- and Maxillofacial Surgery, University Hospital Tuebingen, Osianderstr. 2-8, 72076 Tuebingen, Germany.
Department of Oral and Craniomaxillofacial Surgery, University Medical Center Freiburg, Freiburg, Germany.
J Craniomaxillofac Surg. 2015 Sep;43(7):1177-83. doi: 10.1016/j.jcms.2015.05.020. Epub 2015 Jun 6.
The aim of this work was to analyse the suitability of mesenchymal stem cell isolation by FICOLL density centrifugation and the closed bone marrow aspirate concentrate (BMAC) system for sinus augmentation with bovine bone mineral (BBM) in the sheep model.
16 sheep underwent sinus augmentation with BBM and MSCs; they were divided between two groups with survival points of 8 and 16 weeks. For the FICOLL control arm three, and for the BMAC test arm, five augmentations were performed for each time point. The derived cell numbers were counted; a colony forming unit (CFU) assay was performed; the pluripotency of the MSCs was proved; histological and histomorphometrical analysis were performed.
The approach of using BBM and MSCs in combination with fibrin adhesive was sufficient for new bone formation as the FICOLL experiment indicated. However, due to significantly lower cell numbers isolated using the BMAC in sheep, less new bone was formed in the test arm.
The BMAC system is well suited for human MSC isolation but it needs to be optimized to fit sheep cell characteristics if it is to be used in this animal model.
本研究旨在分析采用Ficoll密度离心法分离间充质干细胞以及使用封闭骨髓抽吸浓缩物（BMAC）系统，在绵羊模型中联合牛骨矿物质（BBM）进行上颌窦提升术的适用性。
16只绵羊接受了使用BBM和间充质干细胞进行的上颌窦提升术；它们被分为两组，存活时间分别为8周和16周。在Ficoll对照组，每个时间点进行3次上颌窦提升术，在BMAC试验组，每个时间点进行5次上颌窦提升术。对获得的细胞进行计数；进行集落形成单位（CFU）分析；证明间充质干细胞的多能性；进行组织学和组织形态计量学分析。
如Ficoll实验所示，将BBM和间充质干细胞与纤维蛋白粘合剂联合使用的方法足以促进新骨形成。然而，由于在绵羊中使用BMAC分离得到的细胞数量显著较少，试验组中形成的新骨较少。
BMAC系统非常适合分离人源间充质干细胞，但如果要在该动物模型中使用，需要进行优化以适应绵羊细胞的特性。
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