不同支架复合人嗅黏膜来源干细胞成骨潜能的评价：一项研究。
Evaluation of the Osteogenic Potential of Different Scaffolds Embedded with Human Stem Cells Originated from Schneiderian Membrane: An Study.
机构信息
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Lebanese University, Beirut, Lebanon.
Laboratory of Cancer Biology and Molecular Immunology, Faculty of Sciences-I, Lebanese University, Hadath- Beirut, Lebanon.
出版信息
Biomed Res Int. 2019 Jan 15;2019:2868673. doi: 10.1155/2019/2868673. eCollection 2019.
BACKGROUND
Novel treatments for bone defects, particularly in patients with poor regenerative capacity, are based on bone tissue engineering strategies which include mesenchymal stem cells (MSCs), bioactive factors, and convenient scaffold supports.
OBJECTIVE
In this study, we aimed at comparing the potential for different scaffolds to induce osteogenic differentiation of human maxillary Schneiderian sinus membrane- (hMSSM-) derived cells. hMSSM-derived cells were seeded on gelatin, collagen, or Hydroxyapatite -Tricalcium phosphate-Fibrin (Ha-TCP-Fibrin) scaffolds. Cell viability was determined using an MTT assay. Alizarin red staining method, Alkaline phosphatase (ALP) activity assay, and quantitative real-time PCR analysis were performed to assess hMSSM-derived cells osteogenic differentiation.
RESULTS
Cell viability, calcium deposition, ALP activity, and osteoblastic markers transcription levels were most striking in gelatin scaffold-embedded hMSSM-derived cells.
CONCLUSION
Our findings suggest a promising potential for gelatin-hMSSM-derived cell construct for treating bone defects.
背景
针对骨缺损的新型治疗方法，特别是针对再生能力差的患者，基于骨组织工程策略，包括间充质干细胞（MSCs）、生物活性因子和方便的支架支持。
目的
在这项研究中，我们旨在比较不同支架在诱导人上颌窦膜源性细胞（hMSSM-）向成骨细胞分化方面的潜力。将 hMSSM 来源的细胞接种在明胶、胶原或羟基磷灰石-磷酸三钙-纤维蛋白（Ha-TCP-Fibrin）支架上。使用 MTT 测定法测定细胞活力。通过茜素红染色法、碱性磷酸酶（ALP）活性测定和实时定量 PCR 分析来评估 hMSSM 来源的细胞成骨分化。
结果
明胶支架中 hMSSM 来源细胞的细胞活力、钙沉积、ALP 活性和成骨细胞标志物转录水平最为显著。
结论
我们的研究结果表明，明胶-hMSSM 衍生细胞构建体在治疗骨缺损方面具有很大的潜力。
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