National Nanotechnology Center, National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani 12120, Thailand.
Department of Manufacturing Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
Int J Pharm. 2015 Aug 15;492(1-2):92-102. doi: 10.1016/j.ijpharm.2015.06.040. Epub 2015 Jul 2.
Nanocarriers based on electrostatic Layer-by-layer (LbL) assembly of CaCO3 nanoparticles (CaCO3 NPs) was investigated. These inorganic nanoparticles was used as templates to construct nanocapsules made from films based on two oppositely charged polyelectrolytes, poly(diallyldimethylammonium chloride), and poly (sodium 4-styrene-sulfonate sodium salt), followed by core dissolution. The naked CaCO3 NPs, CaCO3 NPs coated with the polyelectrolytes and hollow nanocapsules were found with hexagonal shape with average sizes of 350-400 nm. A reversal of the surface charge between positive to negative zeta potential values was found, confirming the adsorption of polyelectrolytes. The loading efficiency and release of curcumin were controlled by the hydrophobic interactions between the drug and the polyelectrolyte matrix of the hollow nanocapsules. The quantity of curcumin released from hollow nanocapsules was found to increase under acidic environments, which is a desirable for anti-cancer drug delivery. The hollow nanocapsules were found to localize in the cytoplasm and nucleus compartment of Hela cancer cells after 24 h of incubation. Hollow nanocapsules were non-toxic to human fibroblast cells. Furthermore, curcumin loaded hollow nanocapsules exhibited higher in vitro cell inhibition against Hela cells than that of free curcumin, suggesting that polyelectrolyte based-hollow nanocapsules can be utilized as new carriers for drug delivery.
基于 CaCO3 纳米粒子（CaCO3 NPs）静电层层（LbL）组装的纳米载体得到了研究。这些无机纳米粒子被用作模板，构建由两种带相反电荷的聚电解质（聚二烯丙基二甲基氯化铵和聚（4-苯乙烯磺酸钠））制成的纳米胶囊，随后溶解核心。裸 CaCO3 NPs、涂覆有聚电解质的 CaCO3 NPs 和中空纳米胶囊被发现为具有平均尺寸为 350-400nm 的六方形状。表面电荷从正到负的 ζ 电位值发生反转，证实了聚电解质的吸附。药物与中空纳米胶囊的聚电解质基质之间的疏水相互作用控制了姜黄素的载药量和释放。在酸性环境下，从中空纳米胶囊中释放的姜黄素的量增加，这是用于抗癌药物递送的理想选择。在孵育 24 小时后，发现中空纳米胶囊定位于 Hela 癌细胞的细胞质和核区室中。中空纳米胶囊对人成纤维细胞无毒。此外，负载姜黄素的中空纳米胶囊对 Hela 细胞的体外细胞抑制作用高于游离姜黄素，表明基于聚电解质的中空纳米胶囊可用作药物递送的新型载体。
