Vollmer G, Helmchen B, Knuppen R
Institut für Biochemische Endokrinologie, Medizinische Universität Lübeck, F.R.G.
J Clin Chem Clin Biochem. 1989 Dec;27(12):953-9. doi: 10.1515/cclm.1989.27.12.953.
We describe two modifications of a double-isotope assay for measuring the concentrations of oestrogen receptors and progesterone receptors in tumour cytosols and extracts of frozen tumour sections and endometrial sections. The concentrations of these receptors are derived from single-point/isoelectric focussing assays after incubation of cytosols or section supernatants either with ([125I]vinyl)-nortestosterone and [3H]oestradiol or [125I]oestradiol and [3H]ORG 2058 (16 alpha-ethyl-21-hydroxy-19-nor[6,7(3)H]pregn-4-ene-3,20-dione). The concentrations of the oestrogen and progesterone receptors found in cytosols (r greater than 0.93) and extracts from sections (r greater than 0.8) by dual label assays are highly correlated with those found by single label assays. The method described represents an approach to the determination of oestrogen and progesterone receptors biochemically in an amount of tissue which is comparable to that needed for immunocytochemical procedures.
我们描述了一种双同位素测定法的两种改进方法，用于测量肿瘤细胞溶质以及冷冻肿瘤切片和子宫内膜切片提取物中雌激素受体和孕激素受体的浓度。这些受体的浓度是在细胞溶质或切片上清液与（[125I]乙烯基）-去甲睾酮和[3H]雌二醇或[125I]雌二醇和[3H]ORG 2058（16α-乙基-21-羟基-19-去甲[6,7(3)H]孕-4-烯-3,20-二酮）孵育后，通过单点/等电聚焦测定法得出的。通过双标记测定法在细胞溶质（r大于0.93）和切片提取物（r大于0.8）中发现的雌激素和孕激素受体浓度与通过单标记测定法发现的浓度高度相关。所描述的方法代表了一种以与免疫细胞化学程序所需组织量相当的组织量，对雌激素和孕激素受体进行生化测定的方法。
