Thornton J G, Wells M
Department of Obstetrics and Gynaecology, University of Leeds.
J Clin Pathol. 1987 Dec;40(12):1437-42. doi: 10.1136/jcp.40.12.1437.
Cryostat sections of unfixed human endometrial tissue from normal, hyperplastic, and carcinomatous endometrium were assayed for oestrogen receptor content using a radiolabelled oestradiol binding assay with isoelectric focusing to separate receptor proteins. The proportions of myometrium, stroma, and glandular tissue were estimated in adjacent sections by point counting. Further cryostat sections were stained for oestrogen receptor immunohistochemically with a monoclonal antibody and the relative staining intensity of the various tissue components assessed. The area proportions and relative immunohistochemical staining intensity of each tissue component in the cryostat sections were then used to adjust the biochemical results and derive separate values of oestrogen receptor content in myometrium, glands, and stroma. The greatest difference in oestrogen receptor content through the menstrual cycle and between normal, hyperplastic, and malignant endometrium occurred in the glandular element.
采用放射性标记雌二醇结合分析法及等电聚焦分离受体蛋白的方法，对来自正常、增生及癌性子宫内膜的未固定人子宫内膜组织的低温恒温器切片进行雌激素受体含量检测。通过点计数法在相邻切片中估算子宫肌层、基质和腺组织的比例。进一步的低温恒温器切片用单克隆抗体进行雌激素受体免疫组织化学染色，并评估各种组织成分的相对染色强度。然后利用低温恒温器切片中各组织成分的面积比例和相对免疫组织化学染色强度来调整生化结果，并得出子宫肌层、腺体和基质中雌激素受体含量的单独值。雌激素受体含量在月经周期中以及正常、增生和恶性子宫内膜之间的最大差异出现在腺成分中。
