Puncher Gregory Neils, Arrizabalaga Haritz, Alemany Francisco, Cariani Alessia, Oray Isik K, Karakulak F Saadet, Basilone Gualtiero, Cuttitta Angela, Mazzola Salvatore, Tinti Fausto
Dept. of Biological, Geological and Environmental Sciences / Laboratory of Genetics and Genomics of Marine Resources and Environment (GenoDREAM), University of Bologna, Ravenna, Italy.
AZTI Tecnalia, Marine Research Division, Herrera Kaia, Pasaia, Gipuzkoa, Spain.
PLoS One. 2015 Jul 6;10(7):e0130407. doi: 10.1371/journal.pone.0130407. eCollection 2015.
The Atlantic bluefin tuna, Thunnus thynnus, is a commercially important species that has been severely over-exploited in the recent past. Although the eastern Atlantic and Mediterranean stock is now showing signs of recovery, its current status remains very uncertain and as a consequence their recovery is dependent upon severe management informed by rigorous scientific research. Monitoring of early life history stages can inform decision makers about the health of the species based upon recruitment and survival rates. Misidentification of fish larvae and eggs can lead to inaccurate estimates of stock biomass and productivity which can trigger demands for increased quotas and unsound management conclusions. Herein we used a molecular approach employing mitochondrial and nuclear genes (CO1 and ITS1, respectively) to identify larvae (n = 188) collected from three spawning areas in the Mediterranean Sea by different institutions working with a regional fisheries management organization. Several techniques were used to analyze the genetic sequences (sequence alignments using search algorithms, neighbour joining trees, and a genetic character-based identification key) and an extensive comparison of the results is presented. During this process various inaccuracies in related publications and online databases were uncovered. Our results reveal important differences in the accuracy of the taxonomic identifications carried out by different ichthyoplanktologists following morphology-based methods. While less than half of larvae provided were bluefin tuna, other dominant taxa were bullet tuna (Auxis rochei), albacore (Thunnus alalunga) and little tunny (Euthynnus alletteratus). We advocate an expansion of expertise for a new generation of morphology-based taxonomists, increased dialogue between morphology-based and molecular taxonomists and increased scrutiny of public sequence databases.
大西洋蓝鳍金枪鱼（Thunnus thynnus）是一种具有重要商业价值的物种，近期遭到了严重的过度捕捞。尽管东大西洋和地中海种群目前已呈现出恢复迹象，但其当前状况仍非常不确定，因此它们的恢复依赖于基于严格科学研究的严格管理。对早期生活史阶段的监测能够依据补充率和存活率，为决策者提供有关该物种健康状况的信息。鱼类幼体和鱼卵的误识别可能导致对种群生物量和生产力的估计不准确，进而引发提高配额的需求以及不合理的管理结论。在此，我们采用一种分子方法，利用线粒体基因和核基因（分别为CO1和ITS1），对由不同机构从地中海三个产卵区收集的188尾幼体进行识别，这些机构与一个区域渔业管理组织合作。我们运用了多种技术来分析基因序列（使用搜索算法进行序列比对、构建邻接树以及基于遗传特征的识别密钥），并对结果进行了广泛比较。在此过程中，我们发现了相关出版物和在线数据库中的各种不准确之处。我们的结果揭示了不同鱼类浮游生物学家采用基于形态学的方法进行分类鉴定时，在准确性方面存在重要差异。虽然所提供的幼体中不到一半是蓝鳍金枪鱼，但其他主要分类单元包括圆舵鲣（Auxis rochei）、长鳍金枪鱼（Thunnus alalunga）和扁舵鲣（Euthynnus alletteratus）。我们主张为新一代基于形态学的分类学家拓展专业知识，加强基于形态学的分类学家与分子分类学家之间的对话，并加强对公共序列数据库的审查。
Zotero 插件
