2012 - 2014年从哥伦比亚卡塔赫纳分离出的B群脑膜炎奈瑟菌菌株的表型和基因型特征
Phenotypic and genotypic characterization of Neisseria meningitidis serogroup B isolates from Cartagena, Colombia, 2012-2014.
作者信息
Moreno Jaime, Sanabria Olga, Saavedra Sandra Yamile, Rodríguez Karina, Duarte Carolina
机构信息
Grupo de Microbiología, Instituto Nacional de Salud, Bogotá, D.C, Colombia.
出版信息
Biomedica. 2015 Jan-Mar;35(1):138-43. doi: 10.1590/S0120-41572015000100017.
INTRODUCTION
Neisseria meningitidis is one of the leading causes of bacterial meningitis. In 2013, an increase in invasive disease caused by N. meningitidis serogroup B occurred in Cartagena, Colombia.
OBJECTIVE
The aim of this study was to characterize serogroup B isolates recovered from patients with meningococcal meningitis in Cartagena during 2012 and 2014.
MATERIALS AND METHODS
Twenty-five isolates were collected through a national, laboratory-based surveillance, and phenotypic and genotypic characteristics were determined by serosubtyping, serotyping, antimicrobial susceptibility testing, repetitive sequence-based PCR (rep-PCR). MLST data available were also used.
RESULTS
The B:10,15:nt phenotype, associated with clonal complex ST-41/44, was responsible for 68.0% of all cases.
CONCLUSION
The clinical, epidemiological and molecular characterization of meningococcal disease is necessary for understanding the spread of virulent meningococcal clones and for facilitating the early detection of cases to reduce epidemic risks.
引言
脑膜炎奈瑟菌是细菌性脑膜炎的主要病因之一。2013年，哥伦比亚卡塔赫纳由B群脑膜炎奈瑟菌引起的侵袭性疾病有所增加。
目的
本研究旨在对2012年至2014年期间在卡塔赫纳从脑膜炎球菌性脑膜炎患者中分离出的B群菌株进行特征分析。
材料与方法
通过基于实验室的全国性监测收集了25株菌株，并通过血清亚型分型、血清分型、抗菌药物敏感性测试、基于重复序列的聚合酶链反应（rep-PCR）确定其表型和基因型特征。还使用了现有的多位点序列分型（MLST）数据。
结果
与克隆复合体ST-41/44相关的B:10,15:nt表型占所有病例的68.0%。
结论
脑膜炎球菌病的临床、流行病学和分子特征分析对于了解毒性脑膜炎球菌克隆的传播以及促进病例的早期发现以降低流行风险是必要的。
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