Chi H I, Otsuka F, Ishibashi Y
Nihon Hifuka Gakkai Zasshi. 1989 Apr;99(4):493-7.
The nuclear DNA content of tumor cells was measured during the radial growth (RGP) and vertical growth (VGP) phases of three cases of superficial spreading melanoma. To compare these two phases, the DNA distribution histogram was analyzed and the DNA index was calculated. A aneuploid population appeared histographically in all specimens of VGP; however, no such abnormal population could be detected in RGP. VGP cells contained greater numbers of polyploid cells and had a higher DNA index value than RGP cells in all cases. These findings support Clark's postulation that the appearance of a new clonal cell population contributes to the development of VGP from RGP in malignant melanoma.
对3例浅表扩散性黑色素瘤在放射状生长（RGP）和垂直生长（VGP）阶段的肿瘤细胞核DNA含量进行了测定。为比较这两个阶段，分析了DNA分布直方图并计算了DNA指数。在所有VGP标本的组织学检查中均出现非整倍体群体；然而，在RGP中未检测到此类异常群体。在所有病例中，VGP细胞所含多倍体细胞数量更多，且DNA指数值高于RGP细胞。这些发现支持了克拉克的假设，即新的克隆细胞群体的出现有助于恶性黑色素瘤从RGP发展为VGP。
