Chi H I, Uyeda Y, Umebayashi Y, Otsuka F
Department of Dermatology, Tokyo Hitachi Hospital, Japan.
Arch Dermatol Res. 1993;285(7):410-4. doi: 10.1007/BF00372134.
The cellular DNA ploidy of 30 cases of malignant melanoma was measured by 4',6-diamidino-2-phenylindole-DNA (DAPI-DNA) microfluorometry. DNA histograms and DNA index values were compared among melanomas of different cell morphology. Epithelioid cell melanomas often showed greater DNA aneuploidy than spindle cell melanomas in terms of histographic pattern. DNA index values of the former (mean +/- standard error, 1.84 +/- 0.31) were significantly higher than those of the latter (1.55 +/- 0.24; P < 0.05). The DNA index values of mixed-type melanomas were intermediate. These results indicate that the epithelioid cell melanomas have greater DNA ploidy abnormalities which are usually correlated with a greater malignant potential in pigmentary neoplasms. Thus our results confirm clinical evidence that melanoma patients with the epithelioid type of cells have a poorer prognosis than those with the spindle type of cells.
采用4',6-二脒基-2-苯基吲哚-DNA（DAPI-DNA）显微荧光测定法对30例恶性黑色素瘤的细胞DNA倍性进行了检测。比较了不同细胞形态的黑色素瘤之间的DNA直方图和DNA指数值。就组织学模式而言，上皮样细胞黑色素瘤通常比梭形细胞黑色素瘤表现出更大的DNA非整倍性。前者的DNA指数值（平均值±标准误，1.84±0.31）显著高于后者（1.55±0.24；P<0.05）。混合型黑色素瘤的DNA指数值介于两者之间。这些结果表明，上皮样细胞黑色素瘤具有更大的DNA倍性异常，这通常与色素性肿瘤中更大的恶性潜能相关。因此，我们的结果证实了临床证据，即上皮样细胞型黑色素瘤患者的预后比梭形细胞型患者更差。
