拟南芥中性神经酰胺酶突变体ncer1积累羟基神经酰胺并对氧化应激敏感。
An Arabidopsis neutral ceramidase mutant ncer1 accumulates hydroxyceramides and is sensitive to oxidative stress.
作者信息
Li Jian, Bi Fang-Cheng, Yin Jian, Wu Jian-Xin, Rong Chan, Wu Jia-Li, Yao Nan
机构信息
State Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou China.
State Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou China ; Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou China.
出版信息
Front Plant Sci. 2015 Jun 19;6:460. doi: 10.3389/fpls.2015.00460. eCollection 2015.
Ceramidases hydrolyze ceramide into sphingosine and fatty acids and, although ceramidases function as key regulators of sphingolipid homeostasis in mammals, their roles in plants remain largely unknown. Here, we characterized the Arabidopsis thaliana ceramidase AtNCER1, a homolog of human neutral ceramidase. AtNCER1 localizes predominantly on the endoplasmic reticulum. The ncer1 T-DNA insertion mutants had no visible phenotype, but accumulated hydroxyceramides, and showed increased sensitivity to oxidative stress induced by methyl viologen. Plants over-expressing AtNCER1 showed increased tolerance to oxidative stress. These data indicate that the Arabidopsis neutral ceramidase affects sphingolipid homeostasis and oxidative stress responses.
神经酰胺酶将神经酰胺水解为鞘氨醇和脂肪酸，尽管神经酰胺酶在哺乳动物中作为鞘脂稳态的关键调节因子发挥作用，但其在植物中的作用仍 largely unknown。在这里，我们对拟南芥神经酰胺酶AtNCER1进行了表征，它是人类中性神经酰胺酶的同源物。AtNCER1主要定位于内质网。 ncer1 T-DNA插入突变体没有可见表型，但积累了羟基神经酰胺，并对甲基紫精诱导的氧化应激表现出更高的敏感性。过表达AtNCER1的植物对氧化应激表现出更高的耐受性。这些数据表明，拟南芥中性神经酰胺酶影响鞘脂稳态和氧化应激反应。
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