Taylor James T, Wang Ken-Der, Horwitz Benjamin, Kolomiets Michael, Kenerley Charles M
Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, United States.
Department of Biology, Technion Israel Institute of Technology, Haifa, Israel.
Front Fungal Biol. 2021 Aug 25;2:718557. doi: 10.3389/ffunb.2021.718557. eCollection 2021.
is a well-known mycoparasitic fungal symbiont that is valued for its biocontrol capabilities. initiates a symbiotic relationship with a plant host through the colonization of its roots. To achieve colonization, the fungus must communicate with the host and evade its innate defenses. In this study, we explored the genes involved with the host communication and colonization process through transcriptomic profiling of the wild-type fungus and selected deletion mutants as they colonized maize roots. Transcriptome profiles of the colonization of maize roots over time revealed that 24 h post inoculation appeared to be a key time for plant-microbe communication, with many key gene categories, including signal transduction mechanisms and carbohydrate transport and metabolism, peaking in expression at this early colonization time point. The transcriptomic profiles of and deletion mutants in the presence of plants demonstrated that , rather than , appears to be the key regulator of the fungal response to maize, with 64% more unique differentially expressed genes compared to . Additionally, we developed a novel algorithm utilizing gene clustering and coexpression network analyses to select potential colonization-related gene targets for characterization. About 40% of the genes identified by the algorithm would have been missed using previous methods for selecting gene targets.
是一种著名的菌寄生真菌共生体，因其生物防治能力而受到重视。它通过定殖植物宿主的根部来建立共生关系。为了实现定殖，真菌必须与宿主进行交流并躲避其固有防御。在本研究中，我们通过对野生型真菌及其选定的缺失突变体在定殖玉米根过程中的转录组分析，探索了与宿主交流和定殖过程相关的基因。随着时间推移玉米根定殖的转录组图谱显示，接种后24小时似乎是植物与微生物交流的关键时间，许多关键基因类别，包括信号转导机制以及碳水化合物运输和代谢，在这个早期定殖时间点表达达到峰值。在有植物存在的情况下对[具体名称1]和[具体名称2]缺失突变体的转录组图谱分析表明，似乎是[具体名称1]而非[具体名称2]是真菌对玉米反应的关键调节因子，与[具体名称2]相比，其独特的差异表达基因多64%。此外，我们开发了一种利用基因聚类和共表达网络分析的新算法，以选择潜在的与定殖相关的基因靶点进行表征。使用该算法鉴定出的基因中，约40%会被以前选择基因靶点的方法遗漏。
