Anjna Babita, Purty Ram Singh
University School of Biotechnology, Guru Gobind Singh Indraprastha University, Sec-16C, Dwarka, New Delhi 110078 India.
Physiol Mol Biol Plants. 2024 Nov;30(11):1795-1818. doi: 10.1007/s12298-024-01527-5. Epub 2024 Nov 23.
Production of stevioside and rebaudioside in is greatly affected due to extreme environmental conditions. MicroRNAs are known to play an important role in post-transcriptional gene regulation. Here, the aim was to study the effect of abiotic stresses on the plantlets and then to identify and validate the expression of the conserved microRNAs and their targets under abiotic stress conditions. The effect of dehydration, salinity and cold stress treatment on 7-week-old plantlets was analyzed. Plant growth, relative water content, malondialdehyde content and antioxidant activity were greatly affected under stress treatment. In the present investigation, amongst the various abiotic stresses studied, 9% PEG treatment greatly affected the plantlets. To identify the microRNAs, BLAST analysis was performed. A homology search of known miRNAs from the PMRD database against non-redundant genomic sequences resulted in the prediction of 37 conserved miRNAs and their targets were identified using the psRNATarget server. All the predicted miRNAs had lengths of 20, 21, 22, 23, 24, and 25 nucleotides, respectively. The identified potential conserved miRNAs belong to 34 distinct miRNA families. The highest potential miRNAs are represented by miR169 family followed by miR156, miR172, and miR396 families. Promoter analysis of miRNA-targets genes revealed the presence of numerous -acting regulatory elements involved in hormonal and stress-response mechanisms. Further, expression analysis revealed an inverse correlation between the selected identified miRNAs and their targets under abiotic stress treatments. Identifying stress-responsive miRNAs and their targets will help us understand the molecular mechanisms of stress tolerance in
The online version contains supplementary material available at 10.1007/s12298-024-01527-5.
由于极端环境条件，甜菊糖苷和莱鲍迪苷的产量受到极大影响。已知微小RNA在转录后基因调控中发挥重要作用。在此，目的是研究非生物胁迫对甜菊幼苗的影响，然后鉴定并验证保守微小RNA及其靶标在非生物胁迫条件下的表达。分析了脱水、盐度和冷胁迫处理对7周龄甜菊幼苗的影响。胁迫处理下，植物生长、相对含水量、丙二醛含量和抗氧化活性受到极大影响。在本研究中，在所研究的各种非生物胁迫中，9%聚乙二醇处理对甜菊幼苗影响极大。为了鉴定微小RNA，进行了BLAST分析。从PMRD数据库中已知的微小RNA与非冗余甜菊基因组序列进行同源性搜索，预测出37个保守微小RNA，并使用psRNATarget服务器鉴定了它们的靶标。所有预测的微小RNA长度分别为20、21、22、23、24和25个核苷酸。鉴定出的潜在保守微小RNA属于34个不同的微小RNA家族。潜在性最高的微小RNA以miR169家族为代表，其次是miR156、miR172和miR396家族。对微小RNA靶标基因的启动子分析揭示了存在众多参与激素和应激反应机制的顺式作用调控元件。此外，表达分析表明在非生物胁迫处理下，所选鉴定出的微小RNA与其靶标之间呈负相关。鉴定胁迫响应性微小RNA及其靶标将有助于我们了解甜菊中胁迫耐受性的分子机制。
在线版本包含可在10.1007/s12298-024-01527-5获取的补充材料。
