Cremmel Clément V M, Zink Christian, Maniura-Weber Katharina, Isa Lucio, Spencer Nicholas D
†Laboratory for Surface Science and Technology, Department of Materials, ETH Zurich, Vladimir-Prelog-Weg 5, CH-8093 Zurich, Switzerland.
§Laboratory for Interfaces, Soft matter and Assembly, Department of Materials, ETH Zurich, Vladimir-Prelog-Weg 5, CH-8093 Zurich, Switzerland.
Langmuir. 2015 Aug 4;31(30):8446-52. doi: 10.1021/acs.langmuir.5b01089. Epub 2015 Jul 20.
Gradient surfaces enable rapid screening and high-throughput investigations in various fields, such as biology and tribology. A new method is described for the preparation of material-independent morphological gradients, in which the density and height of roughness features are varied along two orthogonal axes. A polystyrene-particle-density gradient was produced by a dip-coating process on titanium-oxide-coated silicon wafers. A controlled exposure to ultraviolet light enabled the generation of a particle-height gradient in the orthogonal direction. These gradients were replicated to generate material-independent morphology gradients. MC3T3 cell proliferation studies were performed on titanium-coated replicas and showed a higher cell density on the high-feature-density region of the gradient. The cell area coverage was found to increase with decreasing particle height.
梯度表面能够在生物学和摩擦学等各个领域实现快速筛选和高通量研究。本文描述了一种制备与材料无关的形态梯度的新方法，其中粗糙度特征的密度和高度沿两个正交轴变化。通过浸涂工艺在二氧化钛涂层的硅片上制备了聚苯乙烯颗粒密度梯度。通过控制紫外线照射，在正交方向上产生了颗粒高度梯度。复制这些梯度以生成与材料无关的形态梯度。在钛涂层复制品上进行了MC3T3细胞增殖研究，结果表明在梯度的高特征密度区域细胞密度更高。发现细胞面积覆盖率随颗粒高度的降低而增加。
