囊泡运输。COP 衣被的一种结构和衣被蛋白在膜囊泡组装中的作用。

VESICULAR TRANSPORT. A structure of the COPI coat and the role of coat proteins in membrane vesicle assembly.

机构信息

Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany.

Heidelberg University Biochemistry Center, Heidelberg University, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany.

出版信息

Science. 2015 Jul 10;349(6244):195-8. doi: 10.1126/science.aab1121.

DOI:10.1126/science.aab1121

PMID:26160949

Abstract

Transport of material within cells is mediated by trafficking vesicles that bud from one cellular compartment and fuse with another. Formation of a trafficking vesicle is driven by membrane coats that localize cargo and polymerize into cages to bend the membrane. Although extensive structural information is available for components of these coats, the heterogeneity of trafficking vesicles has prevented an understanding of how complete membrane coats assemble on the membrane. We combined cryo-electron tomography, subtomogram averaging, and cross-linking mass spectrometry to derive a complete model of the assembled coat protein complex I (COPI) coat involved in traffic between the Golgi and the endoplasmic reticulum. The highly interconnected COPI coat structure contradicted the current "adaptor-and-cage" understanding of coated vesicle formation.

摘要

细胞内物质的运输是通过从一个细胞区室出芽并与另一个区室融合的运输小泡来介导的。运输小泡的形成是由膜衣被驱动的，膜衣被将货物定位并聚合成笼状结构来弯曲膜。尽管已经有大量关于这些衣被成分的结构信息，但运输小泡的异质性使得我们无法理解完整的膜衣被如何在膜上组装。我们结合冷冻电镜断层扫描、子断层平均和交联质谱，得出了参与高尔基体和内质网之间运输的组装的衣被蛋白复合物 I（COPI）衣被的完整模型。高度互联的 COPI 衣被结构与当前关于有被小泡形成的“衔接蛋白和笼状结构”理解相矛盾。