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异黄酮可降低帕金森病细胞模型中半胱天冬酶-1和半胱天冬酶-3的水平。

Isoflavone Attenuates the Caspase-1 and Caspase-3 Level in Cell Model of Parkinsonism.

作者信息

Xu Jian-Xin, Song Hai-Ping, Bu Qing-Xia, Feng De-Peng, Xu Xiao-Fan, Sun Qian-Ru, Li Xue-Li

机构信息

Department of Neurology, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong 252000, China.

Department of General Surgery, The Affiliated Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, China.

出版信息

Behav Neurol. 2015;2015:725897. doi: 10.1155/2015/725897. Epub 2015 Jun 16.

DOI:10.1155/2015/725897
PMID:26161002
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4487343/
Abstract

The study has investigated the effect of isoflavone attenuates the caspase-1 and caspase-3 level in cell model of Parkinsonism. The subjects were PC12 cells. They were randomly divided into six groups: control, MPP(+) (250 μmol/L), isoflavone (10 μM), isoflavone (10 μM) + MPP(+) (250 μmol/L), Z-YVAD-CHO (10 nM) + MPP(+) group, and Z-DEVD-CHO (10 nM) + MPP(+) group. Cell viability was measured by MTT methods; the content of tyrosine hydroxylase was measured by immunocytochemistry method of avidinbiotin peroxidase complex; apoptosis ratio was measured by flow cytometry. The results showed that cell viability in the MPP(+) group was lower than in all other five groups. There was no difference in cell viability between isoflavone + MPP(+) and control group. Optical density of TH positive cells in isoflavone group was higher than in control, isoflavone + MPP(+), and MPP(+) only groups. The apoptosis ratio in the isoflavone + MPP(+) group and control group and the Z-YVAD-CHO + MPP(+) and Z-DEVD-CHO + MPP(+) group was similar, which was lower than in the MPP(+) group. The lowest apoptosis ratio was found in the isoflavone only group.

摘要

该研究调查了异黄酮对帕金森病细胞模型中半胱天冬酶 -1和半胱天冬酶 -3水平的影响。实验对象为PC12细胞。它们被随机分为六组：对照组、MPP(+)（250 μmol/L）组、异黄酮（10 μM）组、异黄酮（10 μM）+ MPP(+)（250 μmol/L）组、Z - YVAD - CHO（10 nM）+ MPP(+)组和Z - DEVD - CHO（10 nM）+ MPP(+)组。采用MTT法测定细胞活力；采用抗生物素蛋白 - 生物素过氧化物酶复合物免疫细胞化学法测定酪氨酸羟化酶含量；采用流式细胞术测定凋亡率。结果显示，MPP(+)组的细胞活力低于其他五组。异黄酮 + MPP(+)组与对照组的细胞活力无差异。异黄酮组TH阳性细胞的光密度高于对照组、异黄酮 + MPP(+)组和仅MPP(+)组。异黄酮 + MPP(+)组与对照组以及Z - YVAD - CHO + MPP(+)组和Z - DEVD - CHO + MPP(+)组的凋亡率相似，均低于MPP(+)组。仅异黄酮组的凋亡率最低。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fa5/4487343/2b95eebd71cc/BN2015-725897.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fa5/4487343/c5371232a4dd/BN2015-725897.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fa5/4487343/2b95eebd71cc/BN2015-725897.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fa5/4487343/c5371232a4dd/BN2015-725897.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fa5/4487343/2b95eebd71cc/BN2015-725897.002.jpg

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