Kim Minseong, Kim GeunHyung
Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon, South Korea.
Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon, South Korea.
J Colloid Interface Sci. 2015 Nov 1;457:180-7. doi: 10.1016/j.jcis.2015.07.007. Epub 2015 Jul 7.
Micro/nanofibrous structures have been applied widely in various tissue-engineering applications because the topological structures are similar to the extracellular matrix (ECM), which encourages a high degree of cell adhesion and growth. However, it has been difficult to produce a three-dimensional (3D) fibrous structure using controllable macro-pores. Recently, cellulose has been considered a high-potential natural-origin biomaterial, but its use in 3D biomedical structures has been limited due to its narrow processing window. Here, we suggest a new 3D cellulose scaffold consisting of multi-layered struts made of submicron-sized entangled fibers that were fabricated using an electrohydrodynamic direct jet (EHDJ) process that is spin-printing. By optimizing processing conditions (electric field strength, cellulose feeding rate, and distance between nozzle and target), we can achieve a multi-layered cellulose structure consisting of the cylindrically entangled cellulose fibers. To compare the properties of the fabricated 3D cellulose structure, we used a PCL fibrous scaffold, which has a similar fibrous morphology and pore geometry, as a control. The physical and in vitro biocompatibilities of both fibrous scaffolds were assessed using human dermal fibroblasts, and the cellulose structure showed higher cell adhesion and metabolic activities compared with the control. These results suggest the EHDJ process to be an effective fabricating tool for tissue engineering and the cellulose scaffold has high potential as a tissue regenerative material.
微/纳米纤维结构已被广泛应用于各种组织工程应用中，因为其拓扑结构与细胞外基质（ECM）相似，这有利于高度的细胞黏附和生长。然而，使用可控的大孔来制造三维（3D）纤维结构一直很困难。最近，纤维素被认为是一种具有高潜力的天然生物材料，但其在3D生物医学结构中的应用由于其狭窄的加工窗口而受到限制。在此，我们提出一种新的3D纤维素支架，它由多层支柱组成，这些支柱由亚微米级缠结纤维制成，是使用一种电液动力学直接喷射（EHDJ）工艺即旋转打印制造的。通过优化加工条件（电场强度、纤维素进料速率以及喷嘴与目标之间的距离），我们可以获得一种由圆柱形缠结纤维素纤维组成的多层纤维素结构。为了比较所制造的3D纤维素结构的性能，我们使用了具有相似纤维形态和孔隙几何形状的聚己内酯（PCL）纤维支架作为对照。使用人真皮成纤维细胞评估了两种纤维支架的物理和体外生物相容性，与对照相比，纤维素结构显示出更高的细胞黏附和代谢活性。这些结果表明EHDJ工艺是一种用于组织工程的有效制造工具，并且纤维素支架作为一种组织再生材料具有很高的潜力。
