Ding Nan, Wang Shaobin, Yang Qiong, Li Yongjun, Cheng Hua, Wang Junyun, Wang Dong, Deng Youhui, Yang Yadong, Hu Songnian, Zhao Hua, Fang Xiangdong
CAS Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
CAS Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.
Gene. 2015 Nov 1;572(1):135-145. doi: 10.1016/j.gene.2015.07.013. Epub 2015 Jul 9.
Melanoma is a type of skin cancer that is highly aggressive, and is considered the most deadly of all skin cancers. Currently, there are no effective therapies for melanomas once they undergo metastasis. MicroRNAs (miRNAs) are small, single-stranded, non-coding RNA molecules that can post-transcriptionally regulate gene expression. They have been reported to be associated with the occurrence of many diseases, including human melanoma. However, the mechanisms by which miRNAs exert their effects remain unclear; therefore, a systematic analysis of the miRNAome in human melanoma is necessary. We investigated the miRNAome in human melanocyte and melanoma cell lines using high-throughput RNA sequencing. We identified a group of dysregulated miRNAs by comparing the miRNA expression profiles among the melanoma cell lines. Target genes of these miRNAs encode proteins whose functions are associated with the cell cycle and apoptosis. Gene networks were built to investigate the interactions of genes during melanoma progression. We identified that the key genes that regulate melanoma cell proliferation were regulated by miRNAs. In summary, our investigation of the human melanoma miRNAome using high-throughput sequencing revealed a number of previously unreported miRNAs associated with malignant progression of melanoma. Our findings add to existing knowledge regarding the mechanisms of melanoma development.
黑色素瘤是一种侵袭性很强的皮肤癌，被认为是所有皮肤癌中最致命的。目前，黑色素瘤一旦发生转移，就没有有效的治疗方法。微小RNA（miRNA）是小的单链非编码RNA分子，可在转录后调节基因表达。据报道，它们与包括人类黑色素瘤在内的许多疾病的发生有关。然而，miRNA发挥作用的机制仍不清楚；因此，有必要对人类黑色素瘤中的miRNA组进行系统分析。我们使用高通量RNA测序研究了人类黑素细胞和黑色素瘤细胞系中的miRNA组。通过比较黑色素瘤细胞系之间的miRNA表达谱，我们鉴定出一组失调的miRNA。这些miRNA的靶基因编码的蛋白质功能与细胞周期和细胞凋亡相关。构建基因网络以研究黑色素瘤进展过程中基因之间的相互作用。我们发现调节黑色素瘤细胞增殖的关键基因受miRNA调控。总之，我们使用高通量测序对人类黑色素瘤miRNA组的研究揭示了一些与黑色素瘤恶性进展相关的先前未报道的miRNA。我们的发现增加了关于黑色素瘤发生机制的现有知识。
