miRNA-101a-3p 和 miRNA-144a-3p 对羊驼黑素细胞色素形成的关键基因调控的比较。
Comparison of miRNA-101a-3p and miRNA-144a-3p regulation with the key genes of alpaca melanocyte pigmentation.
机构信息
College of Life Science, Shanxi Agricultural University, Taigu, 030801, China.
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, 100094, China.
出版信息
BMC Mol Biol. 2019 Aug 14;20(1):19. doi: 10.1186/s12867-019-0137-8.
BACKGROUND
Many miRNA functions have been revealed to date. Single miRNAs can participate in life processes by regulating more than one target gene, and more than one miRNA can also simultaneously act on one target mRNA. Thus, a complex regulatory network involved in many processes can be formed. Herein, the pigmentation regulation mechanism of miR-101a-3p and miR-144a-3p was studied at the cellular level by the overexpression and equal overexpression of miR-101a-3p and miR-144a-3p.
RESULTS
Results revealed that miR-101a-3p and miR-144a-3p directly regulated the expression of microphthalmia-associated transcription factor, thereby affecting melanin synthesis.
CONCLUSIONS
The two miRNAs with the same binding site in the same gene independently excreted each other's function. However, the inhibitory effect of miR-144a-3p was stronger than that of miR-101-3p in alpaca melanocytes, although both decreased melanin production.
背景
迄今为止，许多 miRNA 的功能已经被揭示。单个 miRNA 可以通过调节一个以上的靶基因参与生命过程，而一个以上的 miRNA 也可以同时作用于一个靶 mRNA。因此，可以形成涉及许多过程的复杂调控网络。在此，通过 miR-101a-3p 和 miR-144a-3p 的过表达和等表达，在细胞水平上研究了 miR-101a-3p 和 miR-144a-3p 的色素沉着调控机制。
结果
结果表明，miR-101a-3p 和 miR-144a-3p 直接调控小眼畸形相关转录因子的表达，从而影响黑色素合成。
结论
在同一基因中具有相同结合位点的两个 miRNA 独立地分泌彼此的功能。然而，尽管两者都降低了黑色素的产生，但 miR-144a-3p 在羊驼黑素细胞中的抑制作用强于 miR-101-3p。
Zotero 插件