Vongsak Boonyadist, Mangmool Supachoke, Gritsanapan Wandee
Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
Department of Pharmacology, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
Planta Med. 2015 Aug;81(12-13):1084-9. doi: 10.1055/s-0035-1546168. Epub 2015 Jul 10.
The leaves of Moringa oleifera, collected in different provinces in Thailand, were determined for the contents of total phenolics, total flavonoids, major components, and antioxidant activity. The extract and its major active components were investigated for the inhibition of H2O2-induced reactive oxygen species production and the effects on antioxidant enzymes mRNA expression. The extract, crypto-chlorogenic acid, isoquercetin and astragalin, significantly reduced the reactive oxygen species production inducing by H2O2 in HEK-293 cells. Treatment with isoquercetin significantly increased the mRNA expression levels of antioxidant enzymes such as superoxide dismutase, catalase and heme oxygenase 1. These results confirm that M. oleifera leaves are good sources of natural antioxidant with isoquercetin as an active compound.
对采集于泰国不同省份的辣木叶片进行了总酚、总黄酮、主要成分及抗氧化活性含量的测定。研究了提取物及其主要活性成分对H2O2诱导的活性氧生成的抑制作用以及对抗氧化酶mRNA表达的影响。提取物、隐绿原酸、异槲皮苷和紫云英苷显著降低了H2O2诱导的HEK - 293细胞中活性氧的生成。用异槲皮苷处理显著提高了超氧化物歧化酶、过氧化氢酶和血红素加氧酶1等抗氧化酶的mRNA表达水平。这些结果证实辣木叶片是以异槲皮苷为活性化合物的天然抗氧化剂的良好来源。
