辣木在调节糖尿病诱导的氧化应激中的作用。

Role of Moringa oleifera in regulation of diabetes-induced oxidative stress.

机构信息

Alternative Therapeutics Unit, Drug Development Division, Medicinal Research Lab., Department of Chemistry, University of Allahabad, Allahabad-211 002, India.

出版信息

Asian Pac J Trop Med. 2013 Jun;6(6):426-32. doi: 10.1016/S1995-7645(13)60068-1.

DOI:10.1016/S1995-7645(13)60068-1

PMID:23711700

Abstract

OBJECTIVE

To evaluate the antioxidant activity of aqueous extract of Moringa oleifera (M. oleifera) young leaves by in vivo as well as in vitro assays.

METHODS

In vitro study included estimation of total phenolic, total flavonol, total flavonoid and total antioxidant power (FRAP assay). In addition, in vivo study was done with the identified most effective dose of 200 mg/kg of its lyophilized powder on normal and diabetic rats. Its effect on different oxidative free radical scavenging enzymes,viz, superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), lipid peroxide (LPO) contents were measured.

RESULTS

Significant increase in activities of SOD, CAT, GST while, a decrease in LPO content was observed. Whereas, total phenolic, flavonoid and flavonol contents in the extract were found to be 120 mg/g of GAE, 40.5 mg/g of QE and 12.12 mg/g of QE, respectively. On the other hand, FRAP assay results of M. oleifera leaves was (85.00 ± 5.00) μM/g of extract powder.

CONCLUSIONS

The significant antioxidant activities of M. oleifera leaves from both in vivo as well as in vitro studies suggests that the regular intake of its leaves through diet can protect normal as well as diabetic patients against oxidative damage.

摘要

目的

通过体内和体外试验评估辣木（Moringa oleifera）幼叶水提物的抗氧化活性。

方法

体外研究包括测定总酚、总黄酮醇、总类黄酮和总抗氧化能力（FRAP 法）。此外，还对其冻干粉末的鉴定最有效剂量 200mg/kg 进行了体内研究，以观察其对正常和糖尿病大鼠的影响。测定了不同抗氧化自由基清除酶（超氧化物歧化酶[SOD]、过氧化氢酶[CAT]、谷胱甘肽-S-转移酶[GST]）的活性，以及脂质过氧化物（LPO）的含量。