Elatrous Souheil, Ouanes-Besbes Lamia, Ben Sik-Ali Habiba, Hamouda Zineb, BenAbdallah Saoussen, Tilouche Nejla, Jalloul Faten, Fkih-Hassen Mohamed, Dachraoui Fahmi, Ouanes Islem, Abroug Fekri
Laboratoire de Recherche LR12-SP15 (Recherche Cardio - pulmonaire en médecine aigue et Toxicologie), Tunisia; ICU CHU Tahar Sfar. Mahdia, University of Monastir, Tunisia.
Laboratoire de Recherche LR12-SP15 (Recherche Cardio - pulmonaire en médecine aigue et Toxicologie), Tunisia; ICU CHU Fatouma Bourguiba Monastir, University of Monastir, Tunisia.
Toxicon. 2015 Sep 15;104:1-6. doi: 10.1016/j.toxicon.2015.07.003. Epub 2015 Jul 10.
To evaluate the dose-effects of Androctonus australis hector (Aah) venom injected subcutaneously on hemodynamics and neurohormonal secretions, 10 anesthetized and ventilated mongrel dogs, were split in two groups (n = 5/group). Subcutaneous injection was done with either 0.2 mg/kg or 0.125 mg/kg of the purified G50 scorpion toxic fraction. Hemodynamic parameters using right heart catheter were recorded and plasma concentrations of catecholamine, troponin, and serum toxic fraction were measured sequentially from baseline to 120 min. We identified the dose of toxic fraction evoking characteristic hemodynamic perturbation of severe envenomation, the time-lapse to envenomation, and the associated plasma level. The injection of 0.125 mg/kg toxic fraction was not associated with significant variations in hemodynamic parameters, whereas the 0.2 mg/kg dose caused envenomation characterized by significant increase in plasma catecholamines, increased pulmonary artery occluded pressure, mean arterial pressure, and systemic vascular resistance (p < 0.05), in association with sustained decline in cardiac output (p < 0.001). Envenomation occurred by the 30th minute, and the corresponding concentration of toxic fraction was 1.14 ng/ml. The current experiment allowed the identification of the sub-lethal dose (0.2 mg/kg) of the toxic fraction of Aah administered by the subcutaneous route. Two parameters with potential clinical relevance were also uncovered: the time-lapse to envenomation and the corresponding concentration of toxic fraction.
为评估皮下注射澳毒蛛（Androctonus australis hector, Aah）毒液对血流动力学和神经激素分泌的剂量效应，将10只麻醉并通气的杂种犬分为两组（每组n = 5）。分别皮下注射0.2 mg/kg或0.125 mg/kg的纯化G50蝎毒组分。使用右心导管记录血流动力学参数，并依次测量从基线到120分钟的血浆儿茶酚胺、肌钙蛋白浓度以及血清毒组分浓度。我们确定了引起严重中毒特征性血流动力学扰动的毒组分剂量、中毒延迟时间以及相关血浆水平。注射0.125 mg/kg毒组分未引起血流动力学参数的显著变化，而0.2 mg/kg剂量导致中毒，其特征为血浆儿茶酚胺显著增加、肺动脉闭塞压升高、平均动脉压升高和全身血管阻力增加（p < 0.05），同时心输出量持续下降（p < 0.001）。中毒在第30分钟时发生，相应的毒组分浓度为1.14 ng/ml。本实验确定了皮下给药Aah毒组分的亚致死剂量（0.2 mg/kg）。还发现了两个具有潜在临床意义的参数：中毒延迟时间和相应的毒组分浓度。
