Rossi Francesca, Canovetti Annalisa, Malandrini Alex, Lenzetti Ivo, Pini Roberto, Menabuoni Luca
Institute of Applied Physics, Italian National Research Council;
U.O. Oculistica, Nuovo Ospedale Santo Stefano.
J Vis Exp. 2015 Jul 6(101):e52939. doi: 10.3791/52939.
The "all laser" assisted endothelial keratoplasty is a procedure that is performed with a femtosecond laser used to cut the donor tissue at an intended depth, and a near infrared diode laser to weld the corneal tissue. The proposed technique enables to reach the three main goals in endothelial keratoplasty: a precise control in the thickness of the donor tissue; its easy insertion in the recipient bed and a reduced risk of donor lenticule dislocation. The donor cornea thickness is measured in the surgery room with optical coherence tomography (OCT), in order to correctly design the donor tissue dimensions. A femtosecond laser is used to cut the donor cornea. The recipient eye is prepared by manual stripping of the descemetic membrane. The donor endothelium is inserted into a Busin-injector, the peripheral inner side is stained with a proper chromophore (a water solution of Indocyanine Green) and then it is pulled in the anterior chamber. The transplanted tissue is placed in the final and correct location and then diode laser welding is induced from outside the eyeball. The procedure has been performed on more than 15 patients evidencing an improvement in surgery performances, with a good recovery of visual acuity and a reduced donor lenticule dislocation event.
“全激光”辅助内皮角膜移植术是一种手术,该手术使用飞秒激光在预定深度切割供体组织,并使用近红外二极管激光焊接角膜组织。所提出的技术能够实现内皮角膜移植术的三个主要目标:精确控制供体组织的厚度;易于将其插入受体床;降低供体晶状体脱位的风险。在手术室中使用光学相干断层扫描(OCT)测量供体角膜厚度,以便正确设计供体组织尺寸。使用飞秒激光切割供体角膜。通过手动剥离后弹力层来准备受体眼。将供体内皮插入Busin注射器中,其周边内侧用适当的发色团(吲哚菁绿水溶液)染色,然后将其拉入前房。将移植的组织放置在最终的正确位置,然后从眼球外部进行二极管激光焊接。该手术已在15名以上患者身上进行,证明手术效果有所改善,视力恢复良好,供体晶状体脱位事件减少。