Brown Jamin S, Wang Danling, Li Xiaoli, Baluyot Florence, Iliakis Bernie, Lindquist Thomas D, Shirakawa Rika, Shen Tueng T, Li Xingde
Department of Ophthalmology, University of Washington, Seattle, WA 98195, USA.
Cornea. 2008 Aug;27(7):802-10. doi: 10.1097/ICO.0b013e318169d6b7.
To use optical coherence tomography (OCT) as a noninvasive tool to perform in situ characterization of eye bank corneal tissue processed for lamellar keratoplasty.
A custom-built ultrahigh-resolution OCT (UHR-OCT) was used to characterize donor corneal tissue that had been processed for lamellar keratoplasty. Twenty-seven donor corneas were analyzed. Four donor corneas were used as controls, whereas the rest were processed into donor corneal buttons for lamellar transplantation by using hand dissection, a microkeratome, or a femtosecond laser. UHR-OCT was also used to noninvasively characterize and monitor the viable corneal tissue immersed in storage medium over 3 weeks.
The UHR-OCT captured high-resolution images of the donor corneal tissue in situ. This noninvasive technique showed the changes in donor corneal tissue morphology with time while in storage medium. The characteristics of the lamellar corneal tissue with each processing modality were clearly visible by UHR-OCT. The in situ characterization of the femtosecond laser-cut corneal tissue was noted to have more interface debris than shown by routine histology. The effects of the femtosecond laser microcavitation bubbles on the corneal tissue were well visualized at the edges of the lamellar flap while in storage medium.
The results of our feasibility study show that UHR-OCT can provide superb, in situ microstructural characterization of eye bank corneal tissue noninvasively. The UHR-OCT interface findings and corneal endothelial disc thickness uniformity analysis are valuable information that may be used to optimize the modalities and parameters for lamellar tissue processing. The UHR-OCT is a powerful approach that will allow us to further evaluate the tissue response to different processing techniques for posterior lamellar keratoplasty. It may also provide information that can be used to correlate with postoperative clinical outcomes. UHR-OCT has the potential to become a routine part of tissue analysis for any eye bank or centers creating customized lamellar corneal tissue for transplantation.
将光学相干断层扫描（OCT）作为一种非侵入性工具，对用于板层角膜移植的眼库角膜组织进行原位表征。
使用定制的超高分辨率OCT（UHR - OCT）对已处理用于板层角膜移植的供体角膜组织进行表征。分析了27个供体角膜。4个供体角膜用作对照，其余的通过手工剖切、微型角膜刀或飞秒激光处理成用于板层移植的供体角膜纽扣。UHR - OCT还用于非侵入性地表征和监测在储存介质中浸泡3周以上的存活角膜组织。
UHR - OCT原位捕获了供体角膜组织的高分辨率图像。这种非侵入性技术显示了供体角膜组织在储存介质中随时间的形态变化。UHR - OCT可以清晰地看到每种处理方式下板层角膜组织的特征。飞秒激光切割的角膜组织原位表征显示，其界面碎片比常规组织学显示的更多。在储存介质中，飞秒激光微空化气泡对角膜组织的影响在板层瓣边缘清晰可见。
我们的可行性研究结果表明，UHR - OCT可以非侵入性地提供眼库角膜组织出色的原位微观结构表征。UHR - OCT的界面发现和角膜内皮盘厚度均匀性分析是有价值的信息，可用于优化板层组织处理的方式和参数。UHR - OCT是一种强大的方法，将使我们能够进一步评估后板层角膜移植中组织对不同处理技术的反应。它还可能提供可用于与术后临床结果相关联的信息。UHR - OCT有可能成为任何眼库或为移植创建定制板层角膜组织的中心进行组织分析的常规部分。
