荧光团晚期糖基化终产物（AGEs）与烟酰胺腺嘌呤二核苷酸（NADH）的比率是糖尿病慢性肾病和心血管疾病的预测指标。

Fluorophores advanced glycation end products (AGEs)-to-NADH ratio is predictor for diabetic chronic kidney and cardiovascular disease.

作者信息

Ciobanu Dana M, Olar Loredana E, Stefan Razvan, Veresiu Ioan A, Bala Cornelia G, Mircea Petru A, Roman Gabriela

机构信息

"Iuliu Hatieganu" University of Medicine and Pharmacy, Faculty of Medicine, Department of Diabetes, Nutrition and Metabolic Diseases, 2-4 Clinicilor Street, Cluj-Napoca, 400006, Romania.

University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, 3-5 Calea Manastur Street, 400372, Cluj-Napoca, Romania.

出版信息

J Diabetes Complications. 2015 Sep-Oct;29(7):893-7. doi: 10.1016/j.jdiacomp.2015.06.006. Epub 2015 Jun 16.

DOI:10.1016/j.jdiacomp.2015.06.006

PMID:26169772

Abstract

AIMS

An imbalance in advanced glycation end products (AGEs) and NADH formation has been associated with diabetic chronic kidney disease (CKD) and cardiovascular disease (CVD). No data have been reported on simultaneous measurement of AGEs and NADH in type 2 diabetes (T2DM) patients. We aimed to compare AGEs, NADH and the AGEs-to-NADH ratio in T2DM and controls, and to assess its relationship with diabetic CKD and CVD.

MATERIAL AND METHODS

In this cross-sectional study, we measured serum AGEs (370/435nm) and NADH (370/460nm) in T2DM patients (n=63) and controls (n=25) using fluorescence spectroscopy. The AGEs-to-NADH ratio was analyzed according to diabetic CKD and CVD.

RESULTS

We found significantly higher AGEs-to-NADH ratio in T2DM compared to controls. The AGEs-to-NADH ratio was significantly associated with triglycerides, blood glucose, HDL-cholesterol, estimated glomerular filtration rate. The AGEs-to-NADH ratio was a significant predictor for the presence of diabetic CKD and CVD when using ROC curves. Multivariate analysis showed that triglycerides and the presence of T2DM were predictors for the AGEs-to-NADH ratio.

CONCLUSIONS

These findings suggest that the fluorophores AGEs-to-NADH ratio could be a new biomarker for the presence of diabetic CKD and CVD.

摘要

目的

晚期糖基化终产物（AGEs）与烟酰胺腺嘌呤二核苷酸（NADH）生成失衡与糖尿病慢性肾脏病（CKD）及心血管疾病（CVD）相关。尚无关于2型糖尿病（T2DM）患者同时检测AGEs和NADH的报道。我们旨在比较T2DM患者与对照组的AGEs、NADH及AGEs与NADH的比值，并评估其与糖尿病CKD和CVD的关系。

材料与方法

在这项横断面研究中，我们使用荧光光谱法测量了T2DM患者（n = 63）和对照组（n = 25）的血清AGEs（370/435nm）和NADH（370/460nm）。根据糖尿病CKD和CVD分析AGEs与NADH的比值。

结果

我们发现T2DM患者的AGEs与NADH比值显著高于对照组。AGEs与NADH比值与甘油三酯、血糖、高密度脂蛋白胆固醇、估算肾小球滤过率显著相关。使用ROC曲线时，AGEs与NADH比值是糖尿病CKD和CVD存在的显著预测指标。多变量分析显示甘油三酯和T2DM的存在是AGEs与NADH比值的预测因素。