山地风轮菜蜂蜜的抗氧化能力和化学特征：热三烯酚和丁香基衍生物作为生物标志物

Antioxidant capacity and chemical profiles of Satureja montana L. Honey: hotrienol and syringyl derivatives as biomarkers.

作者信息

Jerković Igor, Tuberoso Carlo I G, Marijanović Zvonimir, Kranjac Marina, Malenica-Staver M

机构信息

Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, N. Tesle 10/V, HR-21000 Split, (phone: +385-21-329434; fax: +385-21-329461).

Department of Life and Environmental Sciences, University of Cagliari, via Ospedale 72, IT-09124 Cagliari.

出版信息

Chem Biodivers. 2015 Jul;12(7):1047-56. doi: 10.1002/cbdv.201400183.

DOI:10.1002/cbdv.201400183

PMID:26172325

Abstract

The present study is focused on the antioxidant capacity and chemical profiling of eight Croatian Satureja montana L. honey samples. Among the 20 compounds obtained by headspace solid-phase microextraction (HS-SPME) and identified by GC-FID and GC/MS analyses, hotrienol was predominant (75.9-81.7%). The honey matrix volatile/semivolatile profile was investigated by ultrasonic solvent extraction (USE) followed by GC-FID and GC/MS analyses. The major compounds identified by this latter method were the sinapic-acid derivatives methyl syringate (36.2-72.8%) and syringaldehyde (2.2-43.1%). Direct, targeted HPLC-DAD analyses of the native honey samples revealed the presence of methyl syringate (7.10-39.60 mg/kg) and syringic acid (0.10-1.70 mg/kg). In addition, the total phenolic content of the samples was determined by the FolinCiocalteu assay (311.0-465.9 mg GAE/kg), and the antioxidant capacity was evaluated by the DPPH radical-scavenging activity (0.5-1.0 mmol TEAC/kg) and the ferric reducing antioxidant power (2.5-5.1 mmol Fe(2+) /kg).

摘要

本研究聚焦于八个克罗地亚山地夏枯草蜂蜜样品的抗氧化能力和化学剖析。在通过顶空固相微萃取（HS-SPME）获得并经气相色谱 - 氢火焰离子化检测器（GC-FID）和气相色谱 - 质谱联用仪（GC/MS）分析鉴定出的20种化合物中，热rienol占主导地位（75.9 - 81.7%）。通过超声溶剂萃取（USE），随后进行GC-FID和GC/MS分析，研究了蜂蜜基质的挥发性/半挥发性成分。通过后一种方法鉴定出的主要化合物是芥子酸衍生物丁香酸甲酯（36.2 - 72.8%）和丁香醛（2.2 - 43.1%）。对天然蜂蜜样品进行直接的靶向高效液相色谱 - 二极管阵列检测器（HPLC-DAD）分析，发现存在丁香酸甲酯（7.10 - 39.60 mg/kg）和丁香酸（0.10 - 1.70 mg/kg）。此外，通过福林 - 酚试剂法测定样品的总酚含量（311.0 - 465.9 mg没食子酸当量/kg），并通过DPPH自由基清除活性（0.5 - 1.0 mmol TEAC/kg）和铁还原抗氧化能力（2.5 - 5.1 mmol Fe(2+)/kg）评估抗氧化能力。