卵叶润楠根次生代谢产物的生物学评价
Biological evaluation of secondary metabolites from the root of Machilus obovatifolia.
作者信息
Lin Shan-Yu, Ko Horng-Huey, Lee Shiow-Ju, Chang Hsun-Shuo, Lin Chu-Hung, Chen Ih-Sheng
机构信息
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan 807, R.O.C.
Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan 807, R.O.C.
出版信息
Chem Biodivers. 2015 Jul;12(7):1057-67. doi: 10.1002/cbdv.201400196.
Bioassay-guided fractionation of the root of Machilus obovatifolia led to the isolation of four new lignans, epihenricine B (1), threo-(7'R,8'R) and threo-(7'S,8'S)-methylmachilusol D (2 and 3), and isofragransol A (4), along with 23 known compounds. The compounds were obtained as isomeric mixtures (i.e., 2/3 and 4/20, resp.). The structures were elucidated by spectral analyses. Among the isolates, 1, licarin A (12), guaiacin (14), (±)-syringaresinol (21), and (-)-epicatechin (23) showed ABTS (=2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical-scavenging activity, with SC50 values of 11.7±0.5, 12.3±1.1, 11.0±0.1, 10.6±0.3, and 9.5±0.2 μM in 20 min, respectively. In addition, kachirachirol B (17) showed cytotoxicity against the NCI-H460 cell line with an IC50 value of 3.1 μg/ml.
对倒卵叶润楠根进行生物活性导向的分离，得到了4个新木脂素，表henricine B（1）、苏式-（7′R，8′R）和苏式-（7′S，8′S）-甲基润楠醇D（2和3）以及异芳樟醇A（4），还有23个已知化合物。这些化合物以异构体混合物形式获得（即分别为2/3和4/20）。通过光谱分析确定了其结构。在分离得到的化合物中，1、里卡林A（12）、愈创木酚（14）、（±）-紫丁香树脂醇（21）和（-）-表儿茶素（23）表现出ABTS（=2,2′-偶氮二（3-乙基苯并噻唑啉-6-磺酸）阳离子自由基清除活性，在20分钟内的SC50值分别为11.7±0.5、12.3±1.1、11.0±0.1、10.6±0.3和9.5±0.2 μM。此外，kachirachirol B（17）对NCI-H460细胞系表现出细胞毒性，IC50值为3.1 μg/ml。
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