Bosch Thijs, Pluister Gerlinde N, van Luit Martijn, Landman Fabian, van Santen-Verheuvel Marga, Schot Corrie, Witteveen Sandra, van der Zwaluw Kim, Heck Max E O C, Schouls Leo M
Laboratory for Infectious Diseases & Screening, National Institute for Public Health & the Environment (RIVM), Bilthoven, The Netherlands.
Future Microbiol. 2015;10(7):1155-62. doi: 10.2217/fmb.15.35.
Assess the best approach to type methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcal protein A (spa) typing, multiple-locus variable number tandem repeat analysis (MLVA) or both.
MATERIALS & METHODS: Discriminatory power of spa typing and MLVA was determined using 20,771 MRSA isolates.
There were twice as many MLVA types (MTs) as spa types present in the collection. Among the top 70% of the isolates, 37 spa types and 139 MTs were found. MLVA diversity among the top-10 spa types was high (diversity index 0.96), while spa diversity among the top-10 MTs was much lower (diversity index 0.83). The probability that two MRSA isolates with the same spa type also had the same MT was low (Wallace's coefficient 0.27). By contrast, most MRSA isolates yielding the same MT also had the same spa type (Wallace's coefficient 0.90).
MLVA is superior to spa typing and will suffice to characterize MRSA isolates for surveillance.
评估对耐甲氧西林金黄色葡萄球菌（MRSA）进行分型的最佳方法，即葡萄球菌蛋白A（spa）分型、多位点可变数目串联重复序列分析（MLVA）或两者结合。
使用20771株MRSA分离株确定spa分型和MLVA的鉴别能力。
该集合中存在的MLVA型（MTs）数量是spa型的两倍。在前70%的分离株中，发现了37种spa型和139种MTs。排名前十的spa型之间的MLVA多样性较高（多样性指数0.96），而排名前十的MTs之间的spa多样性则低得多（多样性指数0.83）。具有相同spa型的两个MRSA分离株也具有相同MT的概率较低（华莱士系数0.27）。相比之下，产生相同MT的大多数MRSA分离株也具有相同的spa型（华莱士系数0.90）。
MLVA优于spa分型，足以对MRSA分离株进行特征描述以用于监测。
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