Al-Jawabreh Amer, Dumaidi Kamal, Ereqat Suheir, Nasereddin Abedelmajeed, Al-Jawabreh Hanan, Azmi Kifaya, Al-Laham Nahed, Abdeen Ziad
Arab American University in Jenin, Jenin, Palestine; Al-Quds Public Health Society, Jerusalem, Palestine.
Arab American University in Jenin, Jenin, Palestine.
PLoS Negl Trop Dis. 2015 Jul 16;9(7):e0003934. doi: 10.1371/journal.pntd.0003934. eCollection 2015.
Hydatidosis or echinococcosisis considered a neglected zoonotic disease despite its high burden in the livestock industry and the high risk of infection by humans in endemic areas. In a cross-sectional study we estimated the copro-Incidence and also genotyped Echinococcus granulosus isolates from domestic dogs using polymerase chain reaction (PCR). Medical archives in nine major hospitals in Palestine were reviewed to determine incidence of E. granulosus infection detected in humans during surgery. Faecal samples were collected from 93 domestic dogs in three districts with the highest number of human cases: Al-Khalil (Hebron), Tubas and Jenin. Genomic DNA was extracted from dog faecal samples and amplified by PCR targeting the repeat DNA sequence (EgG1 Hae III) followed by sequencing of five positive samples. Genotyping was determined by sequencing and BLAST searching of mitochondrial cytochrome c oxidase subunit (CO1). The incidence of E. granulosus infection detected in humans at surgery was 1.2 per 100,000 in the West Bank and 1.0 per 100,000 in Gaza Strip. Seventeen of 93 domestic dogs (18%) were positive, based upon comparison with the Echinococcus DNA control. The five sequenced samples were confirmed to be E. granulosus. Successfully genotyped sample belonged to E.granulosus sensu stricto (formerly G1-G3 complex, sheep strain). For domestic dogs, age group (13-24 months) and sex were identified as two risk factors for contracting E. granulosus. The study identified the high incidence of E. granulosus sensu stricto in dogs in Palestine.
尽管包虫病或棘球蚴病在畜牧业中负担沉重，且在流行地区人类感染风险很高，但它仍被视为一种被忽视的人畜共患病。在一项横断面研究中，我们使用聚合酶链反应（PCR）估计了粪检发病率，并对家犬的细粒棘球绦虫分离株进行了基因分型。我们查阅了巴勒斯坦九家主要医院的医学档案，以确定手术期间检测到的人类细粒棘球绦虫感染发病率。在人类病例数最多的三个地区：哈利勒（希布伦）、图巴斯和杰宁，从93只家犬采集了粪便样本。从犬类粪便样本中提取基因组DNA，通过针对重复DNA序列（EgG1 Hae III）的PCR进行扩增，随后对五个阳性样本进行测序。通过对线粒体细胞色素c氧化酶亚基（CO1）进行测序和BLAST搜索来确定基因分型。在约旦河西岸，手术中检测到的人类细粒棘球绦虫感染发病率为每10万人中有1.2例，在加沙地带为每10万人中有1.0例。与棘球绦虫DNA对照相比，93只家犬中有17只（18%）呈阳性。五个测序样本被确认为细粒棘球绦虫。成功进行基因分型的样本属于狭义细粒棘球绦虫（以前的G1 - G3复合体，绵羊株）。对于家犬，年龄组（13 - 24个月）和性别被确定为感染细粒棘球绦虫的两个风险因素。该研究确定了巴勒斯坦家犬中狭义细粒棘球绦虫的高发病率。
